کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7233355 | 1470968 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A microfluidic system integrated with buried optical fibers for detection of Phalaenopsis orchid pathogens
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کلمات کلیدی
RT-LAMPMicrofluidicsPDMSddH2ODNA - DNA یا اسید دزوکسی ریبونوکلئیکdouble-distilled water - آب دوتاییenzyme-linked immunosorbent assays - آنزیم های وابسته به ایمنی جذب شدهdeoxyribonucleic acid - اسید deoxyribonucleicRNA - اسید ریبونوکلئیکribonucleic acid - اسید ریبونوکلئیکELISA - تست الیزاPathogen detection - تشخیص پاتوژنLoop-mediated isothermal amplification - تقویت ایزوترمال متصل به حلقهReverse-transcription loop-mediated isothermal amplification - تقویت ایزوترمال متمرکز حلقه معکوسReverse transcription loop-mediated isothermal amplification - تقویت کننده ایزوترمال متصل به حلقه معکوس معکوسLOD یا Limit of detection - حد تشخیصOptical fibers - فیبر نوریLAMP - لامپlimit of detection - محدودیت تشخیصpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازbase-pair - پایه جفتPolydimethylsiloxane - پلیمتیلسیلوکسان
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Orchids of the genus Phalaenopsis are some of the most economically important plants in Taiwan. Fast, accurate, and on-site detection of pathogens in these orchids is therefore of critical importance in order to prevent or suppress costly disease outbreaks. Traditional pathogen detection methods are time-consuming, require well-equipped laboratories with highly trained personnel, and cannot be conducted in situ. In this study, a microfluidic system integrated with buried optical fibers was developed to detect viral pathogens of Phalaenopsis spp. Briefly, virus-specific ribonucleic acid (RNA) purification was achieved by a pre-treatment incubation with magnetic beads, and reverse-transcription loop-mediated isothermal amplification (RT-LAMP) was used subsequently to amplify the viral RNA. Positive RT-LAMP reactions resulted in the precipitation of magnesium pyrophosphate, which caused a change in turbidity that could be seen by the naked eye. A buried optical fiber-based detection module and a micro-stirring device were then integrated into the microfluidic chip to detect the RT-LAMP reaction product directly on the chip itself by measuring the change in the optical signals caused by the turbidity change associated with a positive amplification. The limit of detection for this system was found to be 25Â fg, which is of similar sensitivity to existing, more laborious methods. Therefore, by using the integrated microfluidic system, a sensitive, rapid, accurate, and automatic diagnosis of viral pathogens in Phalaenopsis spp. orchids could be achieved within only 65Â min.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 63, 15 January 2015, Pages 572-579
Journal: Biosensors and Bioelectronics - Volume 63, 15 January 2015, Pages 572-579
نویسندگان
Chih-Lin Lin, Wen-Hsin Chang, Chih-Hung Wang, Chia-Hwa Lee, Tzong-Yueh Chen, Fuh-Jyh Jan, Gwo-Bin Lee,