کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7281581 | 1473925 | 2014 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immune derived opioidergic inhibition of viscerosensory afferents is decreased in Irritable Bowel Syndrome patients
ترجمه فارسی عنوان
مهار اپی تیوژریکی ایمنی در بیماران مبتلا به سندرم روده تحریک پذیر
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کلمات کلیدی
qRT-PCR[D-Ala2, N-MePhe4, Gly-ol]-enkephalinCVHIBS-CIBS-DTNBSchronic visceral hypersensitivityIBSIBS-AMORMALDI-TOFTrpDRGPBMCNeuro-immuneLPSDAMGO - DREAMPMA - LDC هاOpioid - opioidquantitative RT-PCR - RT-PCR کمیμ-Opioid receptor - μ-گیرنده اپیوئیدTrinitrobenzene sulfonic acid - اسید سولفونیک TrinitrobenzeneHealthy subjects - افراد سالمVisceral pain - درد احشاییPeripheral blood mononuclear cell - سلول تک هسته ای خون محیطیirritable bowel syndrome - سندروم روده تحریک پذیر، کولیت عصبیlipopolysaccharide - لیپوپلی ساکاریدMacrophage - ماکروفاژ matrix-assisted laser desorption/ionization-time of flight - مدت زمان جذب / زمان یونیزاسیون لیزر ماتریس کمک می کندMonocyte - مونوسیتtransient receptor potential - پتانسیل گیرنده گذراdorsal root ganglia - گانگلیس ریشه پشتی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
چکیده انگلیسی
Alterations in the neuro-immune axis contribute toward viscerosensory nerve sensitivity and symptoms in Irritable Bowel Syndrome (IBS). Inhibitory factors secreted from immune cells inhibit colo-rectal afferents in health, and loss of this inhibition may lead to hypersensitivity and symptoms. We aimed to determine the immune cell type(s) responsible for opioid secretion in humans and whether this is altered in patients with IBS. The β-endorphin content of specific immune cell lineages in peripheral blood and colonic mucosal biopsies were compared between healthy subjects (HS) and IBS patients. Peripheral blood mononuclear cell (PBMC) supernatants from HS and IBS patients were applied to colo-rectal sensory afferent endings in mice with post-inflammatory chronic visceral hypersensitivity (CVH). β-Endorphin was identified predominantly in monocyte/macrophages relative to T or B cells in human PBMC and colonic lamina propria. Monocyte derived β-endorphin levels and colonic macrophage numbers were lower in IBS patients than healthy subjects. PBMC supernatants from healthy subjects had greater inhibitory effects on colo-rectal afferent mechanosensitivity than those from IBS patients. The inhibitory effects of PBMC supernatants were more prominent in CVH mice compared to healthy mice due to an increase in μ-opioid receptor expression in dorsal root ganglia neurons in CVH mice. Monocyte/macrophages are the predominant immune cell type responsible for β-endorphin secretion in humans. IBS patients have lower monocyte derived β-endorphin levels than healthy subjects, causing less inhibition of colonic afferent endings. Consequently, altered immune function contributes toward visceral hypersensitivity in IBS.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain, Behavior, and Immunity - Volume 42, November 2014, Pages 191-203
Journal: Brain, Behavior, and Immunity - Volume 42, November 2014, Pages 191-203
نویسندگان
Patrick A. Hughes, Melissa Moretta, Amanda Lim, Dallas J. Grasby, Daniel Bird, Stuart M. Brierley, Tobias Liebregts, Birgit Adam, L. Ashley Blackshaw, Gerald Holtmann, Peter Bampton, Peter Hoffmann, Jane M. Andrews, Heddy Zola, Doreen Krumbiegel,