کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7520591 | 1486860 | 2017 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Oncogenic mutations of PIK3CA and HRAS in carcinoma of cervix in South Indian women
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کلمات کلیدی
PI3KRTK Receptor Tyrosine KinasePCR-CTPPRHEBS6KPIP2GRB2HRASPIK3CAPDGFSOSIRS-1EGFSCCADCRAFmTORAdenocarcinoma - آدنوکارسینوماinsulin receptor substrate-1 - انسولین گیرنده زیربخش 1Bax - باکسOncogenic mutations - جهش های آن کوهنیکیPeripheral blood - خون محیطیCervical cancer - سرطان دهانه رحمepidermal growth factor - عامل رشد اپیدرمیplatelet derived growth factor - فاکتور رشد حاصل از پلاکتFoxO - فاکسوphosphatidylinositol-4,5-bisphosphate - فسفاتیدیلینستول-4،5-بیسفسفاتphosphatidylinositol-3-kinase - فسفاتیدیلینواستیل-3-کینازphosphatidylinositol 4,5-bisphosphate - فسفاتیدیلینوزیتول 4،5-بیسفسفاتrapidly accelerated fibrosarcoma - فیبروسارکوم سریعا شتاب می گیردMethyl ethyl ketone - متیل اتیل کتونMEK - مجاهدین خلقmammalian target of rapamycin - هدف پستانداران رپامایسینpolymerase chain reaction with confronting two-pair primers - واکنش زنجیره ای پلیمراز با مواجهه با آغازگرهای دو جفتBcl-2-associated X protein - پروتئین X مرتبط با Bcl-2protein kinase B - پروتئین کیناز Bmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenmitogen-activated protein kinase kinase - پروتئین کیناز کیناز فعال Mitogen فعالmitogen-activated protein kinase kinase kinase - پروتئین کیناز کیناز کیناز پروتئین فعال Mitogengrowth factor receptor-bound protein 2 - پروتئین گیرنده پروتئینی 2 عامل رشدSquamous cell carcinoma - کارسینوم سلول سنگفرشی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Carcinoma of the cervix is the second most successive reason of female cancer which remains an imperative medical issue in women around the world. Mutations are normally dissected by tissue biopsy which is intrusive, costly and possibly subjective. However, detection of mutations from blood is a non-invasive procedure. However, detection of mutations from blood is a non-invasive procedure. It would offer several advantages, including greater speed and cheap at a cost such that the family members can likewise be screened. Repeated tests after surgery provide an early cautioning for the disease recurrence. The present work aimed to assess the frequency of PIK3CA and HRAS mutations in cervical cancer patients using peripheral blood. This study includes 210 cases presenting cervical cancer and 210 age and sex-matched healthy controls. Genomic DNA was isolated from peripheral blood. The PCR-CTPP method was performed for screening of exons 9 and 20 of PIK3CA and exon 34 HRAS mutations. Sequencing of PIK3CA and of HRAS genes was done for further confirmation. Out of eight mutations studied, no clear disease causing mutations were noticed in any of the cervical cancer patients in the present investigation. Cervical cancer harbors excessive rates of targetable oncogenic mutations. We couldn't find any changes in our investigation. If a connection amongst tissue and non-tissue mutations could be shown, the probability of a basic blood test to distinguish possibility for anticancer treatment comes a bit nearer. Repeated blood tests after surgery provide an early cautioning for disease recurrence. Therefore, in future evaluation of a larger data set and cases with progressive tumor (Stage III-IV) will be required to approve these findings.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Oncological Sciences - Volume 3, Issue 3, December 2017, Pages 112-116
Journal: Journal of Oncological Sciences - Volume 3, Issue 3, December 2017, Pages 112-116
نویسندگان
Geetha kumari Konathala, Ramesh Mandarapu, Sudhakar Godi,