Use of an electrodialytic reactor for the simultaneous β-lactoglobulin enzymatic hydrolysis and fractionation of generated bioactive peptides

► Simultaneous hydrolysis of β-LG and fractionation of generated peptides. ► Simultaneous separation of anionic and cationic peptides generated. ► 15 Anionic peptides were recovered with migration rates between 5.5% and 81.7%. ► The cationic peptide sequence ALPMHIR was recovered with a 66% migration rate. ► First time that hydrolysis was conducted under an electric field.