کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7615376 | 1493991 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantitative determination of cyclic phosphatidic acid and its carba analog in mouse organs and plasma using LC-MS/MS
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کلمات کلیدی
ATXLPALLOQcuprizoneCIDSRMEDTA-Na2AutotaxinCPABSA - BSALC–MS/MS - LC-MS / MSbovine serum albumin - آلبومین سرم گاوethylenediaminetetraacetic acid disodium salt - اتیل سدیم دی سدیم نمک سدیمCollision-induced dissociation - اختلاف ناشی از برخوردcyclic phosphatidic acid - اسید فسفاتیک cycliclysophosphatidic acid - اسید لیسفسفیدیدlower limit of quantification - حد پایین کمیت سنجیCNS - دستگاه عصبی مرکزیLLOD - روزcentral nervous system - سیستم عصبی مرکزیCoefficient of Variation - ضریب تغییرMass spectrometry - طیف سنجی جرمیlower limit of detection - محدودیت پایین تشخیصMultiple sclerosis - مولتیپل اسکلروزیس(ام اس)selected reaction monitoring - نظارت بر واکنش انتخاب شدهliquid chromatography - کروماتوگرافی مایع
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Cyclic phosphatidic acid (cPA), an analog of lysophosphatidic acid, is involved in the regulation of many cellular processes. A sensitive and specific method to quantify the molecular species of cPA is important for studying the physiological and pathophysiological roles of cPA. Here, we developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based quantification method for the simultaneous detection of cPA species having various fatty acids (16:0, 18:0, 18:1, and 18:2) as well as 2-carba-cPA, a chemically synthesized analog of cPA. Chromatography was performed using a reversed-phase C18 column. cPA species were detected using a triple quadrupole mass spectrometer. cPA 17:0 was used as an internal standard. Intra- and interday precision values (CV%) were within 10%. The linear range of detection for each cPA species was 0.01â¯Î¼g/mL to 5â¯Î¼g/mL, with correlation coefficients of 0.998 or higher. The developed method was applied to the quantification of cPA species in mouse plasma and organs. The concentrations of cPA 16:0, 18:0, and 18:1 were revealed to be significantly reduced in the brains of cuprizone-treated mice, a model of multiple sclerosis, compared with control mice. These findings could be important for understanding the roles of cPA in the neurodegenerative processes associated with multiple sclerosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1076, 15 February 2018, Pages 15-21
Journal: Journal of Chromatography B - Volume 1076, 15 February 2018, Pages 15-21
نویسندگان
Yoshibumi Shimizu, Masaki Ishikawa, Mari Gotoh, Keiko Fukasawa, Shinji Yamamoto, Kensuke Iwasa, Keisuke Yoshikawa, Kimiko Murakami-Murofushi,