Determination of nitrofuran metabolites in seafood by ultra high performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry

► A method for the analysis of nitrofuran metabolites by UHPLC-MS/MS was developed. ► Sample treatment includes hydrolysis, derivatization and extraction procedures. ► The method has been validated and good performance characteristics were obtained. ► The method was applied to seafood and nitrofuran metabolites were not found.