Monitoring of adalimumab and antibodies-to-adalimumab levels in patient serum by the homogeneous mobility shift assay

► The authors describe the use of the homogeneous mobility shift assay (HMSA). ► HMSA was used to measure adalimumab and antibodies-to-adalimumab (ATA). ► The HMSAs for adalimumab and ATA have high sensitivity, precision and accuracy. ► Non-responding patients showed high incidence of ATA generation (44%) using HMSA. ► In non-responding patients, levels of adalimumab and ATA were inversely associated.