کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8260564 | 1534664 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Decreased cell proliferation and higher oxidative stress in fibroblasts from Down Syndrome fetuses. Preliminary study
ترجمه فارسی عنوان
کاهش تکثیر سلولی و استرس اکسیداتیو بالاتر در فیبروبلاست ها از جنین سندرم داون. مطالعه مقدماتی
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کلمات کلیدی
GRX1Rcan1DHRMnSODGPXGAPDHCu/Zn superoxide dismutase - Cu / Zn سوپر اکسید دیسموتازROS - ROSTrx1 - TRX1BrdU - بروموداکسی اوریدینbromodeoxyuridine - برومودسوویریدینthioredoxin - تیرودوکسینThioredoxin 1 - تیورودکسین 1dihydrorhodamine - دی هیدرو رودامینDown syndrome - سندرم داونMn superoxide dismutase - سوپر اکسید دیسموتاز MnSuperoxide dismutase - سوکسوکس دیسموتازTelomere length - طول telomereCu/ZnSOD - مس / ZnSODGlutathione - گلوتاتیونglutathione peroxidase - گلوتاتیون پراکسیدازGlutaredoxin 1 - گلوتاراکسین 1glyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
چکیده انگلیسی
Down Syndrome is the most common chromosomal disease and is also known for its decreased incidence of solid tumors and its progeroid phenotype. Cellular and systemic oxidative stress has been considered as one of the Down Syndrome phenotype causes. We correlated, in a preliminary study, the fibroblast proliferation rate and different cell proliferation key regulators, like Rcan1 and the telomere length from Down Syndrome fetuses, with their oxidative stress profile and the Ribonucleic acid and protein expression of the main antioxidant enzymes together with their activity. Increased oxidized glutathione/glutathione ratio and high peroxide production were found in our cell model. These results correlated with a distorted antioxidant shield. The messenger RNA (SOD1) and protein levels of copper/zinc superoxide dismutase were increased together with a decreased mRNA expression and protein levels of glutathione peroxidase (GPx). As a consequence the [Cu/ZnSOD / (catalase + GPx)] activity ratio increases which explains the oxidative stress generated in the cell model. In addition, the expression of thioredoxin 1 and glutaredoxin 1 is decreased. The results obtained show a decreased antioxidant phenotype that correlates with increased levels of Regulator of calcineurin 1 and attrition of telomeres, both related to oxidative stress and cell cycle impairment. Our preliminary results may explain the proneness to a progeroid phenotype.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1842, Issue 1, January 2014, Pages 116-125
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1842, Issue 1, January 2014, Pages 116-125
نویسندگان
Amparo Gimeno, José Luis GarcÃa-Giménez, Laura AudÃ, Nuria Toran, Pilar Andaluz, Francisco DasÃ, José Viña, Federico V. Pallardó,