کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8293042 | 1536741 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Liposome-encapsulated clodronate specifically depletes spinal microglia and reduces initial neuropathic pain
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کلمات کلیدی
BAABulleyaconitine ALECN-methyl-d-aspartic acidDAPINMDA4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولinterleukin - اینترلوکینtumor necrosis factor-α - تومور نکروز عامل αNeuropathic pain - درد نوروپاتیکCNS - دستگاه عصبی مرکزیcentral nervous system - سیستم عصبی مرکزیSpinal cord - طناب نخاعیTNF-α - فاکتور نکروز توموری آلفاMicroglia - میکروگلیاهاGLP-1 receptor - گیرنده GLP-1glucagon-like peptide-1 receptor - گیرنده پپتید-1 مانند گلوکاگون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Liposome-encapsulated clodronate specifically depletes spinal microglia and reduces initial neuropathic pain Liposome-encapsulated clodronate specifically depletes spinal microglia and reduces initial neuropathic pain](/preview/png/8293042.png)
چکیده انگلیسی
Liposome-encapsulated clodronate (LEC) is a specific depletor of macrophages. Our study characterized the LEC depletory effects, given intrathecally, on spinal microglia and assessed its effects on initiation and maintenance of neuropathic pain. Measured by using the MTT assay, LEC treatment specifically inhibited cell viability of cultured primary microglia, but not astrocytes or neurons, from neonatal rats, with an IC50 of 43â¯Î¼g/mL. In spinal nerve ligation-induced neuropathic rats, pretreatment (1 day but not 5 days earlier) with intrathecal LEC specifically depleted microglia (but not astrocytes or neurons) in both contralateral and ipsilateral dorsal horns by the same degree (63% vs. 71%). Intrathecal injection of LEC reversibly blocked the antinociceptive effects of the GLP-1 receptor agonist exenatide and dynorphin A stimulator bulleyaconitine, which have been claimed to be mediated by spinal microglia, whereas it failed to alter morphine- or the glycine receptor agonist gelsemine-induced mechanical antiallodynia which was mediated via the neuronal mechanisms. Furthermore, intrathecal LEC injection significantly attenuated initial (one day after nerve injury) but not existing (2 weeks after nerve injury) mechanical allodynia. Our study demonstrated that LEC, given intrathecally, is a specific spinal microglial inhibitor and significantly reduces initiation but not maintenance of neuropathic pain, highlighting an opposite role of spinal microglia in different stages of neuropathic pain.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 499, Issue 3, 15 May 2018, Pages 499-505
Journal: Biochemical and Biophysical Research Communications - Volume 499, Issue 3, 15 May 2018, Pages 499-505
نویسندگان
Yi-Rui Wang, Xiao-Fang Mao, Hai-Yun Wu, Yong-Xiang Wang,