کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8294333 | 1536751 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differences in TLR7/8 activation between monocytes and macrophages
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کلمات کلیدی
Interleukin-1 receptor-associated kinase 4IRAK1ISGTLR7AP-1IL-12p40IRAK4HEPESTLR8FBSIL-6IRF7cDNA - cDNAComplementary DNA - DNA تکمیلیhydroxyethyl piperazineethanesulfonic acid - اسید هیدروکسی تیلپیرازین اتان سولفونیکinterferon - اینترفرونIFN - اینترفرون هاinterleukin 6 - اینترلوکین 6Enzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاfetal bovine serum - سرم جنین گاوInterferon regulatory factor 7 - عامل تنظیمی اینترفرون 7Macrophages - ماکروفاژها،درشت خوارهاImmune modulation - مدولاسیون ایمنیMonocytes - مونوسیتهاactivator protein 1 - پروتئین فعال کننده 1interleukin-1 receptor-associated kinase 1 - کیناز 1 مرتبط با گیرنده اینترلوکین 1
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The recognition of single-stranded RNA by TLR7/8 leads to the production of NF-κB-mediated cytokines and type I IFNs. However, the role of TLR7/8 activation in monocytes and macrophages is still unclear. The aim of this study was to investigate the differences in the activation of TLR7/8 between these two cell types. Microarray analysis, qRT-PCR and flow cytometry were used to analyse TLR7/8 signalling pathways in monocytes and macrophages after stimulation with agonists. Our data indicated that TLR8 agonists activated the NF-κB- and IRF-mediated pathways in THP-1â¯cells, whereas TLR7 agonists did not. However, silent TLR8 and enhanced TLR7 expression could increase TLR7-induced NF-κB activation in monocytes. TLR7 and TLR8 agonists induced NF-κB activation but no ISG response in PMA-differentiated THP-1â¯cells. The mRNA levels of pro-inflammatory cytokine were elevated upon CL075 stimulation in macrophages compared to monocytes. Thus, TLR7 and TLR8 might modulate different immune responses in monocytes and macrophages.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 497, Issue 1, 26 February 2018, Pages 319-325
Journal: Biochemical and Biophysical Research Communications - Volume 497, Issue 1, 26 February 2018, Pages 319-325
نویسندگان
Hock-Liew Eng, Yuan-Ying Hsu, Tsun-Mei Lin,