کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8295244 | 1536758 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel mouse model for tracking the fate of CXCR5-expressing T cells
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کلمات کلیدی
PD-1C-C chemokine receptor type 7B-cell lymphoma 6ICOSfollicular helper TTFHCXCR5BCL6CCR7OVAOvalbumin - اوبلبومینHumoral immunity - ایمنی هومورالMemory T cells - حافظه T سلول هاFollicular helper T cells - سلول های T کمکی به فولیکول هاGerminal center - مرکز ژرمینالprogrammed cell death 1 - مرگ سلولی برنامه ریزی شده 1Peyer's patches - پیک هاInducible costimulator - کوتیزولاتور قابل انعطاف
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The germinal center (GC) reaction, a critical process in the humoral immune response, requires follicular helper T (Tfh) cells. Tfh cells express the master transcription factor Bcl6 and chemokine receptor CXCR5, which enable them to migrate from the T cell zone to B cell follicles and interact with GC B cells. However, CXCR5 is downregulated when Tfh cells become memory cells. Therefore, it is difficult to track Tfh cells continuously in vivo. In this study, we generated a mouse strain, Cxcr5CreERT2R26Tomato, in which the fluorescent protein tdTomato is inducibly expressed in CXCR5+ cells by tamoxifen administration. After the oral administration of tamoxifen, most Tfh cells in Peyer's patches (PP) from Cxcr5CreERT2R26Tomato mice were tdTomato+. To track antigen-specific Tfh cells in vivo, OVA-specific OT-II T cells derived from Cxcr5CreERT2R26Tomato mice were transferred to wild-type mice, and the recipient mice were immunized with OVA followed by tamoxifen administration. CXCR5+ T cells became tdTomato+ and were mainly located in B cell follicles and GC areas 8 days after immunization. Four weeks after immunization, tdTomato+ OT-II T cells migrated from B cell follicles to the T-B border area and T cell zone after CXCR5 downregulation and CCR7 upregulation. These results indicate that Cxcr5CreERT2R26Tomato mice are a useful tool for studying the cell fate of differentiated Tfh cells in vivo and therefore have implications for the development of therapeutic strategies for infectious and autoimmune diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 495, Issue 2, 8 January 2018, Pages 1642-1647
Journal: Biochemical and Biophysical Research Communications - Volume 495, Issue 2, 8 January 2018, Pages 1642-1647
نویسندگان
Tomo Takebe, Kazuki Sakamoto, Yoshikazu Higami, Yohsuke Harada,