کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8296530 | 1536769 | 2016 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of Cimex lectularius (bedbug) defensin peptide and its antimicrobial activity against human skin microflora
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کلمات کلیدی
NaClRFUMICDefensinEtBrEC50CFUDiSC3(5) - DiSC3 (5)Antibacterial assay - آنتی باکتریالethidium bromide - اتیدیوم برومایدMinimum inhibitory concentration - حداقل غلظت مهاریcircular dichroism - رنگ تابی دورانیBed bug - ساسSodium chloride - سدیم کلریدhalf maximal effective concentration - نیمه حداکثر غلظت موثرRelative Fluorescence Units - واحد فلورسانس نسبیcolony forming units - واحدهای تشکیل دهنده کلنیAntimicrobial peptide - پپتیدهای ضدمیکروبی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Antimicrobial peptides are components of both vertebrate and invertebrate innate immune systems that are expressed in response to exposure to bacterial antigens. Naturally occurring antimicrobial peptides from evolutionarily ancient species have been extensively studied and are being developed as potential therapeutics against antibiotic resistant microorganisms. In this study, a putative Cimex lectularius (bedbug, CL) defensin is characterized for its effectiveness against human skin flora including Gram-negative and Gram-positive bacteria. The bedbug defensin (CL-defensin), belonging to family of insect defensins, is predicted to have a characteristic N-terminal loop, an α-helix, and an antiparallel β-sheet, which was supported by circular dichroism spectroscopy. The defensin was shown to be antimicrobial against Gram-positive bacteria commonly found on human skin (Micrococcus luteus, Corynebacterium renale, Staphylococcus aureus and Staphylococcus epidermidis); however, it was ineffective against common skin Gram-negative bacteria (Pseudomonas aeruginosa and Acinetobacter baumannii) under low-salt conditions. CL-defensin was also effective against M. luteus and C. renale in high-salt (MIC) conditions. Our studies indicate that CL-defensin functions by depolarization and pore-formation in the bacterial cytoplasmic membrane.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 470, Issue 4, 19 February 2016, Pages 955-960
Journal: Biochemical and Biophysical Research Communications - Volume 470, Issue 4, 19 February 2016, Pages 955-960
نویسندگان
Akanksha Kaushal, Kajal Gupta, Monique L. van Hoek,