کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8320589 | 1539393 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Versatility in phospho-dependent molecular recognition of the XRCC1 and XRCC4 DNA-damage scaffolds by aprataxin-family FHA domains
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کلمات کلیدی
FHAAprataxinAPLFXRCC4X-Ray Repair Cross-Complementing Protein 1XRCC1poly(ADP-ribose)polymerase 1DNA-PKcsAPTXBRCTPNKPNTDCK2polynucleotide kinase phosphataseNHEJDNA break repairITCBRCA1 C-terminalPARP-1HIT - اصابتZinc finger - انگشت رویFHA domain - دامنه FHAN-terminal domain - دامنه N-terminalnon-homologous end-joining - عدم پیوستن به همولوگforkhead-associated - فورچون همراه استhistidine triad - هیستیدین سه گانهCasein kinase 2 - کازئین کیناز 2Isothermal titration calorimetry - کالری سنجی تیتاسیون ایزوترمالCoiled-coil - کویل کویل
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Aprataxin, aprataxin and PNKP-like factor (APLF) and polynucleotide kinase phosphatase (PNKP) are key DNA-repair proteins with diverse functions but which all contain a homologous forkhead-associated (FHA) domain. Their primary binding targets are casein kinase 2-phosphorylated forms of the XRCC1 and XRCC4 scaffold molecules which respectively coordinate single-stranded and double-stranded DNA break repair pathways. Here, we present the high-resolution X-ray structure of a complex of phosphorylated XRCC4 with APLF, the most divergent of the three FHA domain family members. This, combined with NMR and biochemical analysis of aprataxin and APLF binding to singly and multiply-phosphorylated forms of XRCC1 and XRCC4, and comparison with PNKP reveals a pattern of distinct but overlapping binding specificities that are differentially modulated by multi-site phosphorylation. Together, our data illuminate important differences between activities of the three phospho-binding domains, in spite of a close evolutionary relationship between them.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 35, November 2015, Pages 116-125
Journal: DNA Repair - Volume 35, November 2015, Pages 116-125
نویسندگان
Amy L. Cherry, Timothy J. Nott, Geoffrey Kelly, Stuart L. Rulten, Keith W. Caldecott, Stephen J. Smerdon,