کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8320658 | 1539403 | 2015 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proteome-wide analysis of SUMO2 targets in response to pathological DNA replication stress in human cells
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کلمات کلیدی
FACSPCNASTRINGDNA replication stressSUMOylationDTTEGTAHCDRPAsearch tool for the retrieval of interacting genes/proteins - ابزار جستجو برای بازیابی ژن / پروتئین های تعامل استethylene glycol tetraacetic acid - اتیلن گلیکول تتراستیک اسیدStreptavidin - استرپتاویدینStrep - استریپhigher-energy collisional dissociation - انحلال تصادفی بالقوه انرژیstable isotope labeling with amino acids in cell culture - برچسب زدن ایزوتوپ پایدار با اسیدهای آمینه در کشت سلولیreplication protein A - تلقیح پروتئین Afluorescence-activated cell sorting - دسته بندی سلول های فعال فلورسنسdithiothreitol - دیتیوتریتولCommon fragile sites - سایت های شکننده مشترکSILAC - سیلکMass spectrometry - طیف سنجی جرمیhuman influenza hemagglutinin - هموگلوبینین آنفلوانزا انسانpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کارا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
SUMOylation is a form of post-translational modification involving covalent attachment of SUMO (Small Ubiquitin-like Modifier) polypeptides to specific lysine residues in the target protein. In human cells, there are four SUMO proteins, SUMO1-4, with SUMO2 and SUMO3 forming a closely related subfamily. SUMO2/3, in contrast to SUMO1, are predominantly involved in the cellular response to certain stresses, including heat shock. Substantial evidence from studies in yeast has shown that SUMOylation plays an important role in the regulation of DNA replication and repair. Here, we report a proteomic analysis of proteins modified by SUMO2 in response to DNA replication stress in S phase in human cells. We have identified a panel of 22 SUMO2 targets with increased SUMOylation during DNA replication stress, many of which play key functions within the DNA replication machinery and/or in the cellular response to DNA damage. Interestingly, POLD3 was found modified most significantly in response to a low dose aphidicolin treatment protocol that promotes common fragile site (CFS) breakage. POLD3 is the human ortholog of POL32 in budding yeast, and has been shown to act during break-induced recombinational repair. We have also shown that deficiency of POLD3 leads to an increase in RPA-bound ssDNA when cells are under replication stress, suggesting that POLD3 plays a role in the cellular response to DNA replication stress. Considering that DNA replication stress is a source of genome instability, and that excessive replication stress is a hallmark of pre-neoplastic and tumor cells, our characterization of SUMO2 targets during a perturbed S-phase should provide a valuable resource for future functional studies in the fields of DNA metabolism and cancer biology.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 25, January 2015, Pages 84-96
Journal: DNA Repair - Volume 25, January 2015, Pages 84-96
نویسندگان
Sara Bursomanno, Petra Beli, Asif M. Khan, Sheroy Minocherhomji, Sebastian A. Wagner, Simon Bekker-Jensen, Niels Mailand, Chunaram Choudhary, Ian D. Hickson, Ying Liu,