کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8323187 | 1539890 | 2014 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A proteomic screen with Drosophila Opa1-like identifies Hsc70-5/Mortalin as a regulator of mitochondrial morphology and cellular homeostasis
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کلمات کلیدی
OMMAMPMitofilinTORCtetramethyl rhodamine methyl esterNMDAN-methyl-d-aspartateCyt CCCCPHtrA2TMRMDrp-1opa1 - grandpa1optic atrophy 1 - آتروفی اپتیکی 1Autophagy - اتوفاژیimm - انحصارinner mitochondrial membrane - درونی غشای میتوکندریcytochrome c - سیتوکروم سیouter mitochondrial membrane - غشای بیرونی میتوکندریFusion - فیوژنLysosome - لیزوزومCell death - مرگ سلولی Mortalin - مورتالینMitochondrial morphology - مورفولوژی میتوکندریDrosophila melanogaster - مگس سرکه، مگس میوهCarbonylcyanide m-chlorophenylhydrazone - کربوکیل سایینید m-chlorophenylhydrazone
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Mitochondrial morphology is regulated by conserved proteins involved in fusion and fission processes. The mammalian Optic atrophy 1 (OPA1) that functions in mitochondrial fusion is associated with Optic Atrophy and has been implicated in inner membrane cristae remodeling during cell death. Here, we show Drosophila Optic atrophy 1-like (Opa1-like) influences mitochondrial morphology through interaction with 'mitochondria-shaping' proteins like Mitochondrial assembly regulatory factor (Marf) and Drosophila Mitofilin (dMitofilin). To gain an insight into Opa1-like's network, we delineated bonafide interactors like dMitofilin, Marf, Serine protease High temperature requirement protein A2 (HTRA2), Rhomboid-7 (Rho-7) along with novel interactors such as Mortalin ortholog (Hsc70-5) from Drosophila mitochondrial extract. Interestingly, RNAi mediated down-regulation of hsc70-5 in Drosophila wing imaginal disc's peripodial cells resulted in fragmented mitochondria with reduced membrane potential leading to proteolysis of Opa1-like. Increased ecdysone activity induced dysfunctional fragmented mitochondria for clearance through lysosomes, an effect enhanced in hsc70-5 RNAi leading to increased cell death. Over-expression of Opa1-like rescues mitochondrial morphology and cell death in prepupal tissues expressing hsc70-5 RNAi. Taken together, we have identified a novel interaction between Hsc70-5/Mortalin and Opa1-like that influences cellular homeostasis through mitochondrial fusion.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 54, September 2014, Pages 36-48
Journal: The International Journal of Biochemistry & Cell Biology - Volume 54, September 2014, Pages 36-48
نویسندگان
Shamik Banerjee, Balaji Chinthapalli,