کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8336590 | 1540636 | 2016 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The delta 6 desaturase knock out mouse reveals that immunomodulatory effects of essential n-6 and n-3 polyunsaturated fatty acids are both independent of and dependent upon conversion
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کلمات کلیدی
EPAΔ6-desaturaseD6DTLRAPCALATCrLPSFBSArachidonic acid - اسید آراشیدونیکalpha-linolenic acid - اسید آلفا لینولنیکEicosapentaenoic acid - اسید ایکوزاپنتانوئیکdocosahexaenoic acid - اسید داکوزاگزوائونیکLinoleic acid - اسید لینولئیکfatty acid desaturase 2 - اسید چرب دارونما 2Polyunsaturated fatty acid - اسید چرب غیر اشباعinflammation - التهاب( توروم) Toll-like receptor - تیالآرDHA - دوکوساهگزائنوئیک اسیدFish oil - روغن ماهیlong chain - زنجیره طولانیfetal bovine serum - سرم جنین گاوantigen presenting cell - سلولهای پردازنده آنتیژنCytokines - سیتوکین هاphycoerythrin - فایکوئیریدینlipopolysaccharide - لیپوپلی ساکاریدFatty acid metabolism - متابولیسم اسید چربmajor histocompatibility complex - مجموعه سازگاری بافتی اصلیMHC - مجموعه سازگاری بافتی اصلیT-cell receptor - گیرنده لنفوسیت T
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The delta 6 desaturase knock out mouse reveals that immunomodulatory effects of essential n-6 and n-3 polyunsaturated fatty acids are both independent of and dependent upon conversion The delta 6 desaturase knock out mouse reveals that immunomodulatory effects of essential n-6 and n-3 polyunsaturated fatty acids are both independent of and dependent upon conversion](/preview/png/8336590.png)
چکیده انگلیسی
Typically fatty acids (FA) exert differential immunomodulatory effects with n-3 [α-linolenic acid (ALA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)] and n-6 [linoleic acid (LA) and arachidonic acid (AA)] exerting anti- and pro-inflammatory effects, respectively. This over-simplified interpretation is confounded by a failure to account for conversion of the parent FA (LA and ALA) to longer-chain bioactive products (AA and EPA/DHA, respectively), thereby precluding discernment of the immunomodulatory potential of specific FA. Therefore, we utilized the Î6-desaturase model, wherein knockout mice (D6KO) lack the Fads2 gene encoding for the rate-limiting enzyme that initiates FA metabolism, thereby providing a model to determine specific FA immunomodulatory effects. Wild-type (WT) and D6KO mice were fed one of four isocaloric diets differing in FA source (9 weeks): corn oil (LA-enriched), arachidonic acid single cell oil (AA-enriched), flaxseed oil (ALA-enriched) or menhaden fish oil (EPA/DHA-enriched). Splenic mononuclear cell cytokine production in response to lipopolysaccharide (LPS), T-cell receptor (TCR) and anti-CD40 stimulation was determined. Following LPS stimulation, AA was more bioactive compared to LA, by increasing inflammatory cytokine production of IL-6 (1.2-fold) and TNFα (1.3-fold). Further, LPS-stimulated IFNγ production in LA-fed D6KO mice was reduced 5-fold compared to LA-fed WT mice, indicating that conversion of LA to AA was necessary for cytokine production. Conversely, ALA exerted an independent immunomodulatory effect from EPA/DHA and all n-3 FA increased LPS-stimulated IL-10 production versus LA and AA. These data definitively identify specific immunomodulatory effects of individual FA and challenge the simplified view of the immunomodulatory effects of n-3 and n-6 FA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Nutritional Biochemistry - Volume 32, June 2016, Pages 29-38
Journal: The Journal of Nutritional Biochemistry - Volume 32, June 2016, Pages 29-38
نویسندگان
Jennifer M. Monk, Danyelle M. Liddle, Daniel J.A. Cohen, Denis H. Tsang, Lyn M. Hillyer, Salma A. Abdelmagid, Manabu T. Nakamura, Krista A. Power, David W.L. Ma, Lindsay E. Robinson,