کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8337851 | 1540969 | 2018 | 28 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Duplicated membrane estrogen receptors in the European sea bass (Dicentrarchus labrax): Phylogeny, expression and regulation throughout the reproductive cycle
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کلمات کلیدی
GSIGnRHNuclear estrogen receptorGPER7TMGPCRs18SESRqPCR17β-estradiol - 17β استرادیولEF1α - EF1aG protein-coupled estrogen receptor - G پروتئین گیرنده استروژن همراه استG-protein coupled receptors - G-پروتئین گیرنده های متصل شده18S ribosomal RNA - RNA ریبوزوم 18SAmino acids - اسید آمینه یا آمینو اسیدcoding sequence - توالی کدگذاریReproduction - تولید مثل یا زادآوریgonadosomatic index - شاخص گنادوسوماتیکelongation factor 1-alpha - ضریب انحنای 1-آلفاPituitary - غدهٔ زیرمغزی یا هیپوفیزEvolution - فرگشت Teleost - ماهی پیوسته استخوانHPG axis - محور HPGBrain - مغزGonadotropin-releasing hormone - هورمون آزاد کننده گنادوتروپینfollicle stimulating hormone - هورمون تحریک کننده فولیکولluteinizing hormone - هورمون جسم زردFSH - هورمون محرکه فولیکولی quantitative polymerase chain reaction - واکنش زنجیره ای پلیمراز کمیCdS - کادمیم سولفید، سولفید کادمیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The numerous estrogen functions reported across vertebrates have been classically explained by their binding to specific transcription factors, the nuclear estrogen receptors (ERs). Rapid non-genomic estrogenic responses have also been recently identified in vertebrates including fish, which can be mediated by membrane receptors such as the G protein-coupled estrogen receptor (Gper). In this study, two genes for Gper, namely gpera and gperb, were identified in the genome of a teleost fish, the European sea bass. Phylogenetic analysis indicated they were most likely retained after the 3R teleost-specific whole genome duplication and raises questions about their function in male and female sea bass. Gpera expression was mainly restricted to brain and pituitary in both sexes while gperb had a widespread tissue distribution with higher expression levels in gill filaments, kidney and head kidney. Both receptors were detected in the hypothalamus and pituitary of both sexes and significant changes in gpers expression were observed throughout the annual reproductive season. In female pituitaries, gpera showed an overall increase in expression throughout the reproductive season while gperb levels remained constant. In the hypothalamus, gpera had a higher expression during vitellogenesis and decreased in fish entering the ovary maturation and ovulation stage, while gperb expression increased at the final atresia stage. In males, gpers expression was constant in the hypothalamus and pituitary throughout the reproductive cycle apart from the mid- to late testicular development stage transition when a significant up-regulation of gpera occurred in the pituitary. The differential sex, seasonal and subtype-specific expression patterns detected for the two novel gper genes in sea bass suggests they may have acquired different and/or complementary roles in mediating estrogens actions in fish, namely on the neuroendocrine control of reproduction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 178, April 2018, Pages 234-242
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 178, April 2018, Pages 234-242
نویسندگان
PatrÃcia I.S. Pinto, André R. Andrade, M. Dulce Estêvão, M. Victoria Alvarado, Alicia Felip, Deborah M. Power,