کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8338951 | 1541012 | 2013 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Testosterone implants modify the steroid hormone balance and the gonadal physiology of gilthead seabream (Sparus aurata L.) males
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
HSIDMRT1TGFB1IL1bRCSCYP11B1DPITLRTNFaSELEEE2CCl4ISMCYP11A1AGS17α-ethinyl estradiolCyp19a1aaromatase geneHsd3bmAbGSIDHTPCNAIgM11KT11-ketotestosterone - 11-کتواستاسترون17β-estradiol - 17β استرادیولEDCs - EDC هاMonoclonal antibody - آنتی بادی مونوکلونالProliferating Cell Nuclear Antigen - آنتیژن هسته ای تکثیر سلولیSteroidogenic enzymes - آنزیم های استروئیدوژنیکimmunoglobulin M - ایمونوگلوبولین MTestis - بیضهtestosterone - تستوسترونTUNEL - تونلBody mass - جرم بدنGilthead seabream - جلبک دریاییOHT - خطراتDihydrotestosterone - دی هیدروتستوسترونStar - ستارهSignificance level - سطح اهمیتHepatosomatic index - شاخص Hepatosomaticgonadosomatic index - شاخص گنادوسوماتیکLeukocytes - لکوسیتEndocrine Disrupting Chemicals - مواد شیمیایی خرابکار غدد درون ریزbody weight - وزن بدنReproductive cycles - چرخه تولید مثلacidophilic granulocytes - گرانولوسیت اسیدوفیلیکAndrogen Receptor - گیرنده آندروژنی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Androgens can induce complete spermatogenesis in immature or prepubertal teleost fish; however, many aspects of the role of androgens in adult teleost spermatogenesis remain elusive. We used the in situ forming microparticle (ISM) system containing 1 mg of testosterone (T)/kg body weight (T-ISM) in a homogenous population of gilthead seabream at testicular involution stage to study in vivo the effects of T on the sex steroid hormone balance and on the physiology of the gilthead seabream gonad. The levels of T, 11-ketotestosterone (11KT) and 17β-estradiol (E2) in plasma, gonad and liver were determined in T-ISM implanted specimens after 7, 14, 21 and 28 days. The effect of T-ISM was evaluated on (i) de novo synthesis and metabolism of T in the gonad and liver by measuring the gene expression levels of the main steroidogenic proteins involved, (ii) the progress of spermatogenesis, (iii) the presence of different leukocyte cell types in the gonad, and (iv) the mRNA expression of some genes involved in the leukocyte migratory influx into the gonad and of some immune-relevant molecules. T-ISM implants promote an increase of T up to supra-physiological levels which induce a depletion of E2 levels and maintain the 11KT levels at physiological concentrations. The gene expression profile of some steroidogenic enzymes in gonad and liver ruled out the transformation of T into estrogenic compounds following T-ISM implantation. Moreover, androgens may also be involved in the leukocyte migratory influx, which occurred even when cytokine, chemokine and cell adhesion molecule gene expressions were down-regulated. Moreover, T-ISM implants block germ cell proliferation, although increased dmrt1 gene expression may prevent the complete depletion of germ cells in the gonad. Furthermore, T down-regulated the expression of several tlr genes, which may result in the inhibition of the immune response in the gonad through the impaired ability to recognize and respond to pathogens.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 138, November 2013, Pages 183-194
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 138, November 2013, Pages 183-194
نویسندگان
Miriam Sánchez-Hernández, Elena Chaves-Pozo, Isabel Cabas, Victoriano Mulero, Alfonsa GarcÃa-Ayala, Alicia GarcÃa-Alcázar,