کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8339100 | 1541012 | 2013 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Adiponectin activates the AMPK signaling pathway to regulate lipid metabolism in bovine hepatocytes
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کلمات کلیدی
ChREBPcarbohydrate responsive element-binding proteinSCD-1ACCαAdipoR1NEBacyl-CoA oxidaseCPT-1AMPKL-FABPPPARαSREBP-1cADIPOR2FASACOAMP-activated protein kinase - AMP-پروتئین کیناز فعال شده استadiponectin - آدیپونکتینElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزacetyl-CoA carboxylase α - اتیل کوآ آر کربوکسیلااز αStearoyl-CoA desaturase 1 - استارویل-کاپا دساتوراز 1fatty acid synthase - اسید چرب سنتازLipid oxidation - اکسیداسیون لیپیدیtriglyceride - تریگلیسریدNegative energy balance - تعادل انرژی منفیLipid synthesis - سنتز لیپیدEMSA یا electrophoretic mobility shift assay - سنجش تغییر تحرک الکتروفورتیکLiver fatty acid-binding protein - پروتئین مرتبط با اسید چرب کبدیsterol regulatory element-binding protein 1c - پروتئین وابسته به استرول تنظیم کننده پروتئین 1ccarnitine palmitoyltransferase 2 - کاردینیت پالمیتویل ترانسفراز 2Carnitine palmitoyltransferase 1 - کارنتین پالمیتویل ترانسفراز 1adiponectin receptor 1 - گیرنده adiponectin 1adiponectin receptor 2 - گیرنده آدوبونکتین 2peroxisome proliferator-activated receptor α - گیرنده پروتئینی فعال پروکسایزوم α
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Adiponectin (Ad) plays a crucial role in hepatic lipid metabolism. However, the regulating mechanism of hepatic lipid metabolism by Ad in dairy cows is unclear. Hepatocytes from a newborn female calf were cultured in vitro and treated with different concentrations of Ad and BML-275 (an AMPKα inhibitor). The results showed that Ad significantly increased the expression of two Ad receptors. Furthermore, the phosphorylation and activity of AMPKα, as well as the expression levels and transcriptional activity of peroxisome proliferator activated receptor-α (PPARα) and its target genes involved in lipid oxidation, showed a corresponding trend of upregulation. However, the expression levels and transcriptional activity of sterol regulatory element binding protein 1c (SREBP-1c) and carbohydrate-responsive element-binding protein (ChREBP) decreased in a similar manner. When BML-275 was added, the p-AMPKα level as well as the expression and activity of PPARα and its target genes were significantly decreased. However, the expression levels of SREBP-1c, ChREBP and their target genes showed a trend of upregulation. Furthermore, the triglyceride (TG) content was significantly decreased in the Ad-treated groups. These results indicate that Ad activates the AMPK signaling pathway and mediates lipid metabolism in bovine hepatocytes cultured in vitro by promoting lipid oxidation, suppressing lipid synthesis and reducing hepatic lipid accumulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 138, November 2013, Pages 445-454
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 138, November 2013, Pages 445-454
نویسندگان
Hui Chen, Liang Zhang, Xinwei Li, Xiaobing Li, Guoquan Sun, Xue Yuan, Liancheng Lei, Juxiong Liu, Liheng Yin, Qinghua Deng, Jianguo Wang, Zhaoxi Liu, Wentao Yang, Zhe Wang, Hui Zhang, Guowen Liu,