کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8344595 | 1541584 | 2018 | 50 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Polysulfides and products of H2S/S-nitrosoglutathione in comparison to H2S, glutathione and antioxidant Trolox are potent scavengers of superoxide anion radical and produce hydroxyl radical by decomposition of H2O2
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کلمات کلیدی
5-(diethoxyphosphoryl)-5-methyl-1-pyrroline N-oxidenitrosopersulfideDEPMPOS-nitrosoglutathioneGSNOGSH(±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid - (±) -6-هیدروکسی-2،5،7،8-تترامکتیل کروم-2-کربوکسیلیک اسید2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide - 2- (4-کاربوکسی فنیل) -4،4،5،5-تترامتییلیمیدازولین-1-اکسیل -3 اکسیدcPTIO - eptirROS - ROSAntioxidant - آنتی اکسیدانDNA cleavage - تجزیه DNATrolox - ترولکسElectron paramagnetic resonance - تشدید پارامغناطیس الکترونEPR - تشدید پارامغناطیس الکترونHydroxyl radical - رادیکال هیدروکسیلSulfide - سولفیدSuperoxide - سوپر اکسیدEPR spectroscopy - طیف سنجی EPRNitric oxide - نیتریک اکسیدGlutathione - گلوتاتیونReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Exogenous and endogenously produced sulfide derivatives, such as H2S/HSâ/S2â, polysulfides and products of the H2S/S-nitrosoglutathione interaction (S/GSNO), affect numerous biological processes in which superoxide anion (O2â) and hydroxyl (OH) radicals play an important role. Their cytoprotective-antioxidant and contrasting pro-oxidant-toxic effects have been reported. Therefore, the aim of our work was to contribute to resolving this apparent inconsistency by studying sulfide derivatives/free radical interactions and their consequent biological effects compared to the antioxidants glutathione (GSH) and Trolox. Using the electron paramagnetic resonance (EPR) spin trapping technique and O2â, we found that a polysulfide (Na2S4) and S/GSNO were potent scavengers of O2â and cPTIO radicals compared to H2S (Na2S), GSH and Trolox, and S/GSNO scavenged the DEPMPO-OH radical. As detected by the EPR spectra of DEPMPO-OH, the formation of OH in physiological solution by S/GSNO was suggested. All the studied sulfide derivatives, but not Trolox or GSH, had a bell-shaped potency to decompose H2O2 and produced OH in the following order: S/GSNO > Na2S4 â¥Â Na2S > GSH = Trolox = 0, but they scavenged OH at higher concentrations. In studies of the biological consequences of these sulfide derivatives/H2O2 properties, we found the following: (i) S/GSNO alone and all sulfide derivatives in the presence of H2O2 cleaved plasmid DNA; (ii) S/GSNO interfered with viral replication and consequently decreased the infectivity of viruses; (iii) the sulfide derivatives induced apoptosis in A2780 cells but inhibited apoptosis induced by H2O2; and (iv) Na2S4 modulated intracellular calcium in A87MG cells, which depended on the order of Na2S4/H2O2 application. We suggest that the apparent inconsistency of the cytoprotective-antioxidant and contrasting pro-oxidant-toxic biological effects of sulfide derivatives results from their time- and concentration-dependent radical production/scavenging properties and their interactions with O2â, OH and H2O2. The results imply a direct involvement of sulfide derivatives in O2â and H2O2/OH free radical pathways modulating antioxidant/toxic biological processes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nitric Oxide - Volume 76, 1 June 2018, Pages 136-151
Journal: Nitric Oxide - Volume 76, 1 June 2018, Pages 136-151
نویسندگان
Anton Misak, Marian Grman, Zuzana Bacova, Ingeborg Rezuchova, Sona Hudecova, Elena Ondriasova, Olga Krizanova, Vlasta Brezova, Miroslav Chovanec, Karol Ondrias,