کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8358552 | 1542073 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation of an alcohol dehydrogenase cDNA from and characterization of its expression in chrysanthemum under waterlogging
ترجمه فارسی عنوان
جداسازی سندرم الیدیدیدروژناز و مشخص نمودن بیان آن در گل داودی در زیر غرق شدن
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کلمات کلیدی
PDCADHqRT-PCR1-MCPPCD1-methylcyclopropene - 1-متیل سیکلوپروپینEthylene - اتیلنAlcohol dehydrogenase - الکل دهیدروژنازGene expression - بیان ژنPyruvate decarboxylase - دیربوکسیلاز پیرواتWaterlogging - غرق شدنProgrammed cell death - مرگ برنامهریزی شده یاختهquantitative reverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی معکوس و پلیمریزا معکوسChrysanthemum - گل داودی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
چکیده انگلیسی
A PCR strategy was used to isolate a full-length CgADH (alcohol dehydrogenase) cDNA from chrysanthemum. The gene putatively encodes a 378 residue polypeptides, which shares 95% homology with tomato alcohol dehydrogenase class III. Endogenous ethylene generated in waterlogged Chrysanthemum zawadskii was enhanced by exogenous ethylene but decreased by 1-methylcyclopropene (1-MCP), an inhibitor of ethylene action. In waterlogged roots, the transcription of the gene encoding alcohol dehydrogenase (ADH, EC 1.1.1.1) increased rapidly but transiently, peaking at 7.5 fold the non-waterlogged level after 2 h of stress. Waterlogging elevated ADH activity after a prolonged episode of stress. The exogenous supply of 40 μL Lâ1 ethylene suppressed the production of ethanol, while that of 4 μL Lâ1 1-MCP enhanced it. Ethylene appeared to suppress an acceleration of both CgADH expression and fermentation, and alleviates ethanolic fermentation probably through by as a signal to acceleration of waterlogging-induced aerenchyma formation. This supports the previously observed phenomenon that the expression level of ADH gene is regulated by the local level of physiologically active ethylene. The relevance of the CgADH gene in relation to chrysanthemum waterlogging was discussed as well.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 212, November 2013, Pages 48-54
Journal: Plant Science - Volume 212, November 2013, Pages 48-54
نویسندگان
Dongmei Yin, Dian Ni, Lili Song, Zhiguo Zhang,