کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8360920 | 1542361 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Plasma-activated medium selectively eliminates undifferentiated human induced pluripotent stem cells
ترجمه فارسی عنوان
محیط فعال پلاسما به طور انتخابی سلول های بنیادی پلوروپتوت القا شده توسط انسان را بی خاصیت می کند
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کلمات کلیدی
Human induced pluripotent stem cells (hiPSCs)CATNHDFsHPSCsGPx1hiPSCsPAMhESCsataxia telangiectasia mutated - Ataxia telangiectasia جهش یافته استROS - ROSOxidative stress - تنش اکسیداتیوATM - خودپردازRONS - رونSOD - سدHuman induced pluripotent stem cells - سلول های بنیادی تکامل یافته القا شده توسط انسانhuman embryonic stem cells - سلول های بنیادی جنینی انسانHuman pluripotent stem cells - سلول های بنیادی پلورپوفتون انسانیSuperoxide dismutase - سوکسوکس دیسموتازnormal human dermal fibroblasts - فیبروبلاست های طبیعی پوست انسانplasma-activated medium - محیط فعال پلاسماPropidium iodide - پروتئین یدیدRegenerative medicine - پزشکی ترمیمیCatalase - کاتالازglutathione peroxidase 1 - گلوتاتیون پراکسیداز 1Reactive oxygen/nitrogen species - گونه های واکنشی اکسیژن / نیتروژنReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Human pluripotent stem cells, including human induced pluripotent stem cells (hiPSCs), are promising materials for regenerative medicine and cell transplantation therapy. However, tumorigenic potential of residual undifferentiated stem cells hampers their use in these therapies. Therefore, it is important to develop methods that selectively eliminate undifferentiated stem cells from a population of differentiated cells before their transplantation. In the present study, we investigated whether plasma-activated medium (PAM) selectively eliminated undifferentiated hiPSCs by inducing external oxidative stress. PAM was prepared by irradiating cell culture medium with non-thermal atmospheric pressure plasma. We observed that PAM selectively and efficiently killed undifferentiated hiPSCs cocultured with normal human dermal fibroblasts (NHDFs), which were used as differentiated cells. We also observed that undifferentiated hiPSCs were more sensitive to PAM than hiPSC-derived differentiated cells. Gene expression analysis suggested that lower expression of oxidative stress-related genes, including those encoding enzymes involved in hydrogen peroxide (H2O2) degradation, in undifferentiated hiPSCs was one of the mechanisms underlying PAM-induced selective cell death. PAM killed undifferentiated hiPSCs more efficiently than a medium containing the same concentration of H2O2 as that in PAM, suggesting that H2O2 and various reactive oxygen/nitrogen species in PAM selectively eliminated undifferentiated hiPSCs. Thus, our results indicate that PAM has a great potential to eliminate tumorigenic hiPSCs from a population of differentiated cells and that it may be a very useful tool in regenerative medicine and cell transplantation therapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Regenerative Therapy - Volume 5, December 2016, Pages 55-63
Journal: Regenerative Therapy - Volume 5, December 2016, Pages 55-63
نویسندگان
Ryo Matsumoto, Kazunori Shimizu, Takunori Nagashima, Hiromasa Tanaka, Masaaki Mizuno, Fumitaka Kikkawa, Masaru Hori, Hiroyuki Honda,