کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8452254 1547700 2017 23 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Culture methods of diffuse intrinsic pontine glioma cells determine response to targeted therapies
ترجمه فارسی عنوان
روش های کشت سلول های گلیوما پنتین در حال پخش، واکنش به درمان های هدفمند را مشخص می کنند
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
چکیده انگلیسی
Diffuse intrinsic pontine glioma (DIPG) is an aggressive type of brainstem cancer occurring mainly in children, for which there currently is no effective therapy. Current efforts to develop novel therapeutics for this tumor make use of primary cultures of DIPG cells, maintained either as adherent monolayer in serum containing medium, or as neurospheres in serum-free medium. In this manuscript, we demonstrate that the response of DIPG cells to targeted therapies in vitro is mainly determined by the culture conditions. We show that particular culture conditions induce the activation of different receptor tyrosine kinases and signal transduction pathways, as well as major changes in gene expression profiles of DIPG cells in culture. These differences correlate strongly with the observed discrepancies in response to targeted therapies of DIPG cells cultured as either adherent monolayers or neurospheres. With this research, we provide an argument for the concurrent use of both culture conditions to avoid false positive and false negative results due to the chosen method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 360, Issue 2, 15 November 2017, Pages 397-403
نویسندگان
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