کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8485363 | 1551732 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Intra-seasonal antibody repertoire analysis of a subject immunized with an MF59®-adjuvanted pandemic 2009 H1N1 vaccine
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
RBSPBS-TTMBMBCCVVIGHVECxHRPRHADPBSclonal lineageHCDR3BCRSHMPBMCmAbcandidate vaccine virusFACSRAGs3,3′,5,5′-tetramethylbenzidine - 3،3 '، 5،5'-تترامیلیل بنزیدینBSA - BSAbovine serum albumin - آلبومین سرم گاوamino acid - آمینو اسیدMonoclonal antibody - آنتی بادی مونوکلونالRecombinant antigens - آنتی ژن های بازسازی شدهInfluenza - آنفلوانزا somatic hypermutation - ابرمتن سوتیRNA - اسید ریبونوکلئیکimmunoglobulin - ایمونوگلوبولینUca - بالاfluorescence-activated cell sorting - دسته بندی سلول های فعال فلورسنسRoom temperature - دمای اتاقreceptor-binding site - سایت گیرنده گیرندهMemory B cell - سلول B حافظهPeripheral blood mononuclear cell - سلول تک هسته ای خون محیطیdulbecco’s phosphate-buffered saline - فسفات باسیل نمک dulbeccoimmunoglobulin heavy chain variable region - منطقه متغیر زنجیره سنگین ایمونوگلوبولینneuraminidase - نورآمینیدازNucleotide - نوکلئوتیدhemagglutinin - هماگلوتینینRecombinant hemagglutinin - هموگلوتینین بازآفرینیVaccination - واکسیناسیونAntibody - پادتَن یا آنتیبادیHorseradish peroxidase - پراکسیداز هوررادیشB cell receptor - گیرنده سلول B
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
During the height of the 2009 H1N1 swine-derived influenza pandemic, a clinical trial was conducted in which seven subjects were immunized using a monovalent, MF59®-adjuvanted vaccine, developed from an egg-passaged candidate vaccine virus (CVV), A/California/07/2009 X-181. Whole blood was collected prior to immunization and at 8, 22, and 202â¯days post-vaccination, and subjects' serological responses were evaluated. Here, we reconstruct and examine the longitudinal, influenza-specific circulating B cell repertoire of one subject in that study. Genotypic analysis of 390 total subject-derived antibodies (Abs) revealed a total of 29 germline genes in use among immunoglobulin heavy chain variable regions (IgHV), with the majority of those sequences isolated representing memory recall responses and two major lineages dominating the early response. In vitro phenotyping showed a diverse set of binding epitopes on the surface glycoproteins hemagglutinin (HA) and neuraminidase (NA), many of which are considered subdominant. Strong correlations were found between IgHV germline usage among non-related lineages and both binding epitope and neutralization breadth. Results here highlight the potential for Ab responses to be misdirected to egg-adaptive artifacts on CVVs while simultaneously stressing the ability to mount potent, broadly neutralizing responses to mostly novel antigens via recall of subdominant memory responses, as well as the need for evaluating alternative endpoint assays and anti-NA responses following clinical trials.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 36, Issue 35, 23 August 2018, Pages 5325-5332
Journal: Vaccine - Volume 36, Issue 35, 23 August 2018, Pages 5325-5332
نویسندگان
Jack Ferdman, Giuseppe Palladino, Hua-Xin Liao, M. Anthony Moody, Thomas B. Kepler, Giuseppe Del Giudice, Philip R. Dormitzer, Stephen C. Harrison, Ethan C. Settembre, Pirada Suphaphiphat,