کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8507499 | 1555963 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transcriptomic analysis of Ehrlichia ruminantium during the developmental stages in bovine and tick cell culture
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کلمات کلیدی
RT-qPCRRPKMtRNATh1T4SStmRNAReverse transcription qPCREhrlichia ruminantiumRNA-seqNGSrRNAqPCRCTLcDNA - cDNAComplementary DNA - DNA تکمیلیDNA - DNA یا اسید دزوکسی ریبونوکلئیکQuantitative PCR - PCR کمیRibosomal RNA - RNA RibosomalT-helper 1 - T helper 1cytotoxic T-lymphocytes - T-lymphocytes سیتوتوکسیکTandem repeats - Tandem تکرار می شودTRPs - TRP هاdeoxyribonucleic acid - اسید deoxyribonucleicribonucleic acid - اسید ریبونوکلئیکRNA - اسید ریبونوکلئیکtransfer RNA - انتقال RNAinterferon - اینترفرونIFN - اینترفرون هاelementary body - بدن ابتداییReticulate body - بدن تلطیف شدهTranscriptome - ترانسکریپتومRNA sequencing - ترتیب RNAfold change - تغییر در برابرNext generation sequencing - توالی نسل بعدیedge - حاشیه، غیرمتمرکزType IV secretion system - سیستم ترشحی نوع IVpost infection - عفونت پستThreshold value - مقدار آستانه
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The use of bioinformatics tools to search for possible vaccine candidates has been successful in recent years. In an attempt to search for additional vaccine candidates or improve the current heartwater vaccine design, a genome-wide transcriptional profile of E. ruminantium (Welgevonden strain) replicating in bovine endothelial cells (BA886) and Ixodes scapularis embryonic tick cells (IDE8) was performed. The RNA was collected from the infective extracellular form, the elementary bodies (EBs) and vegetative intracellular form, reticulate bodies (RBs) and was used for transcriptome sequencing. Several genes previously implicated with adhesion, attachment and pathogenicity were exclusively up-regulated in the EBs from bovine and tick cells. Similarly, genes involved in adaptation or survival of E. ruminantium in the host cells were upâregulated in the RBs from bovine cells. Thus, it was concluded that those genes expressed in the EBs might be important for infection of mammalian and tick host cells and these may be targets for both cell and humoral mediated immune responses. Alternatively, those exclusively expressed in the RBs may be important for survival in the host cells. Exported or secreted proteins exclusively expressed at this stage are ideal targets for the stimulation of cytotoxic T-lymphocyte (CTL) immune responses in the host.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ticks and Tick-borne Diseases - Volume 9, Issue 1, January 2018, Pages 126-134
Journal: Ticks and Tick-borne Diseases - Volume 9, Issue 1, January 2018, Pages 126-134
نویسندگان
Mabotse A. Tjale, Alri Pretorius, Antoinette Josemans, Mirinda Van Kleef, Junita Liebenberg,