کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8531142 | 1559731 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The mechanisms of Ag85A DNA vaccine activates RNA sensors through new signal transduction
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
IRF3MAVSAg85AIRF7dsRBDRIG-IMDA-5BMDCsPRRAPCPAMPsAntigen presentation - ارائه آنتیژنpathogen-associated molecular patterns - الگوهای مولکولی مرتبط با پاتوژنImmunogenicity - ایمنی زاییantigen presenting cell - سلولهای پردازنده آنتیژنmitochondrial antiviral signaling - سیگنالینگ ضد ویروسی میتوکندریInterferon regulatory factor 3 - عامل تنظیمی اینترفرون 3Interferon regulatory factor 7 - عامل تنظیمی اینترفرون 7caspase activation and recruitment domain - فعال سازی caspase و دامنه استخدامSignal transduction - هدایت سیگنالDNA vaccines - واکسن های DNAretinoic acid-inducible gene I - ژن I القاء شده اسید رتینوئیکCARD - کارتpattern recognition receptor - گیرنده شناسایی الگو
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The mechanisms of Ag85A DNA vaccine activates RNA sensors through new signal transduction The mechanisms of Ag85A DNA vaccine activates RNA sensors through new signal transduction](/preview/png/8531142.png)
چکیده انگلیسی
Low immunogenicity is one of the major problems limiting the clinical use for DNA vaccines, which makes it impossible to obtain a strong protective immune response after vaccination. In order to explore whether Ag85A DNA vaccine could mount more efficiently protective immune response through new RNA sensor and its signal transduction pathway of antigen presentation we designed and synthesized Ag85A gene fragment containing multiple points mutations and transfected the gene fragment into the dendritic cell line (DC2.4) by CRISPR/Cas9. Subsequently, we focused on the changes of RNA sensors RIG-I, Mda-5, and the downstream adaptors MAVS, IRF3, IRF7 and IFN-β. The results indicated the significant increases in the mRNA and protein expression of RNA sensors RIG-I, Mda-5 and related adaptors MAVS, IRF3, IRF7, and IFN-β in the mutant DC 2.4 cells. The flow cytometry results demonstrated that the expression of MHC II on the surface of DC 2.4 significantly increased when compared with that in control. Therefore, it is suggested that Ag85A mutant DNA could release immunogenic message through RNA sensors and related adaptors via non protein pathway. There is at least one RNA signal transduction pathway of Ag85A DNA in DC2.4 cell. The work provides a new mode of action for nucleic acid vaccine to improve immunogenicity and meaningful data for the better understanding of the mechanisms of DNA vaccine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 59, June 2018, Pages 1-11
Journal: International Immunopharmacology - Volume 59, June 2018, Pages 1-11
نویسندگان
Jingbo Zhai, Qiubo Wang, Yunfeng Gao, Ran Zhang, Shengjun Li, Bing Wei, Yong You, Xun Sun, Changlong Lu,