کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8545663 | 1561583 | 2016 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mechanism of apoptosis induction in human breast cancer MCF-7 cell by Ruviprase, a small peptide from Daboia russelii russelii venom
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کلمات کلیدی
EMEMPBSRVVp53DICPARPGSHHPBLqRT-PCRBSA - BSAROS - ROSbovine serum albumin - آلبومین سرم گاوRussell's viper venom - جاذبه روسل راسEagle's minimal essential medium - حداقل مواد ضروری عقابApoptosis - خزان یاختهایPhosphate buffered saline - فسفات بافر شورHuman peripheral blood lymphocytes - لنفوسیت های خون محیطی انسانMitochondrial pathway - مسیر میتوکندریاییquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیPropidium iodide - پروتئین یدیدpoly (ADP-ribose) polymerase - پلی (ADP-ribose) پلیمرازAnticancer peptide - پپتید ضد سرطانCaspase-7 - کاسپاز 7differential interference contrast - کنتراست تداخل دیفرانسیلReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Ruviprase, a 4.4 kDa peptide isolated from Daboia russelii russelii venom demonstrated antiproliferative activity against EMT6/AR1, U-87MG, HeLa and MCF-7 cancer cells with an IC50 value of 23.0, 8.8, 5.8 and 4.0 μg mlâ1, respectively. However, it was nontoxic to non-cancerous human embryonic kidney cell and human peripheral blood lymphocytes. Flow-cytometric analysis confirmed the apoptosis induction in MCF-7 cells by Ruviprase where it induced DNA condensation but did not cause mitotic blockage or chromosomal aberration in treated-cells. Immunofluorescence microscopic analysis indicated Ruviprase induced apoptosis in MCF-7 cells through p53 and p21-mediated pathways. Ruviprase generated reactive oxygen species (ROS), altered the mitochondrial transmembrane potential, and significantly decreased the cellular glutathione (GSH) content of MCF-7 cells. Immunoblotting and quantitative real-time PCR (qRT-PCR) analyses suggested that Ruviprase down-regulated the expression of anti-apoptotic protein Bcl-2, increased cleavage of poly (ADP-ribose) polymerase (PARP) protein, and up-regulated the expression of pro-apoptotic protein Bax, as well as executer protein caspase-7 to induced apoptosis in MCF-7 cells via intrinsic pathway. This is the first report on the characterization of the anticancer potential of a small, non-toxic and anticoagulant peptide purified from Russell's viper venom.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemico-Biological Interactions - Volume 258, 25 October 2016, Pages 297-304
Journal: Chemico-Biological Interactions - Volume 258, 25 October 2016, Pages 297-304
نویسندگان
Rupamoni Thakur, Sudarshan Kini, Sillarine Kurkalang, Atanu Banerjee, Purba Chatterjee, Abhishek Chanda, Anupam Chatterjee, Dulal Panda, Ashis K. Mukherjee,