کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8646241 | 1570073 | 2018 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Deep sequencing of small RNAs reveals ribosomal origin of microRNAs in Oryza sativa and their regulatory role in high temperature
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کلمات کلیدی
RDNAqRT-PCRPectin esteraseN22rRNARibosomal DNA - DNA ریبوزومیRibosomal RNA - RNA RibosomalSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNAHigh temperature stress - استرس درجه حرارت بالاRice - برنجGene expression - بیان ژنquantitative real time PCR - زمان واقعی PCR کمیMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAGlycosyltransferase - گلیکوزیلتransferase
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Deep sequencing of small RNAs reveals ribosomal origin of microRNAs in Oryza sativa and their regulatory role in high temperature Deep sequencing of small RNAs reveals ribosomal origin of microRNAs in Oryza sativa and their regulatory role in high temperature](/preview/png/8646241.png)
چکیده انگلیسی
MicroRNAs are small noncoding regulatory RNAs which control gene expression by mRNA degradation or translational repression. They are significant molecular players regulating important biological processes such as developmental timing and stress response. We report here the discovery of miRNAs derived from ribosomal DNA using the small RNA datasets of 16 deep sequencing libraries of rice. Twelve putative miRNAs were identified based on highly stringent criteria of novel miRNA prediction. Surprisingly, 10 putative miRNAs (mi_7403, mi_8435, mi_12675, mi_4266, mi_4758, mi_4218, mi_8200, mi_4644, mi_14291, mi_16235) originated from rDNA of rice chromosome 9. Expression analysis of putative miRNAs and their target genes in heat tolerant and susceptible rice cultivars in control and high temperature treated seedlings revealed differential regulation of rDNA derived miRNAs. This is the first report of rDNA derived miRNAs in rice which indicates their role in gene regulation during high temperature stress in plants. Further studies in this area will open new research challenges and opportunities to broaden our knowledge on gene regulation mechanisms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene Reports - Volume 11, June 2018, Pages 270-278
Journal: Gene Reports - Volume 11, June 2018, Pages 270-278
نویسندگان
Satendra K. Mangrauthia, B. Sailaja, Madhu Pusuluri, Biswajit Jena, Vishnu V. Prasanth, Surekha Agarwal, P. Senguttuvel, N. Sarla, V. Ravindra Babu, Desiraju Subrahmanyam, S.R. Voleti,