کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8646250 | 1570074 | 2018 | 35 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Spatial and temporal expression of bmp8a and its role in regulation of lipid metabolism in zebrafish Danio rerio
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کلمات کلیدی
tRNAkDaqRT-PCRIPTGBCAPAGEhpfDPFPFATRISPTUmAbSDSbicinchonininc acidPBS1-phenyl-2-thioureacDNA - cDNADNA complementary to RNA - DNA مکمل RNADNA - DNA یا اسید دزوکسی ریبونوکلئیکkiloDalton(s) - kiloDalton (s)monoclonal Ab - Monoclonal Abdeoxyribonucleic acid - اسید deoxyribonucleicRNA - اسید ریبونوکلئیکribonucleic acid - اسید ریبونوکلئیکPA-gel electrophoresis - الکتروفورز PA-geltransfer RNA - انتقال RNAisopropyl β-d-thiogalactoside - ایزوپروپیل β-d-thiogalactosidedays post fertilization - بارور شدن روزانهtris (hydroxymethyl) aminomethane - تریس (هیدروکسی متیل) آمینومتانMin - حداقلminute - دقیقهRoom temperature - دمای اتاقMelting Temperature - دمای ذوبSecond - دومینHour - ساعتhours post fertilization - ساعت پس از لقاحsodium dodecyl sulfate - سدیم دودسیل سولفاتLuria-Bertani (medium) - لوریا بارتانی (متوسط)Lipid metabolism - متابولیسم لیپیدPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریBMP - مدیریت فرایند کسب و کارObesity - مرض چاقیnucleotide(s) - نوکلئوتید (ها)polymerase chain reaction - واکنش زنجیره ای پلیمرازquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیPCR - واکنش زنجیرهٔ پلیمرازparaformaldehyde - پارافرمالدهیدpotential of hydrogen - پتانسیل هیدروژنBone morphogenetic protein - پروتئین مورفوژنیک استخوانZebrafish - گورخرماهی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Spatial and temporal expression of bmp8a and its role in regulation of lipid metabolism in zebrafish Danio rerio Spatial and temporal expression of bmp8a and its role in regulation of lipid metabolism in zebrafish Danio rerio](/preview/png/8646250.png)
چکیده انگلیسی
Bone morphogenetic proteins (BMP) are members of the transforming growth factor beta (TGF-β) superfamily. BMP8a and BMP8b have different functions in mammals. However, only one bmp8 gene is present in zebrafish Danio rerio, so we are interested to investigate the function and evolution of this gene. The zebrafish bmp8 gene was identified to represent the bmp8a ortholog (zbmp8a) but not the bmp8b ortholog. In adult zebrafish, the zbmp8a mRNA was highly expressed in the tissues of intestine, testis, skin and brain; during embryogenesis, zbmp8a was present in all cells at early stages and then mainly restricted to the head region and intestine at late stages. Furthermore, the zbmp8a expression was decreased during fasting and increased dramatically after refeeding. In high-fat diet induced obese zebrafish, the expression of zbmp8a was up-regulated significantly. In addition, temperature and salinity affected the transcript levels of zbmp8a. Importantly, the recombinant mature peptide of zBmp8a was administered by microinjection to yolk sac of 3-dpf larvae, resulting that the lipidolysis was accelerated. Besides, zBmp8a protein increased the expression levels of pgc-1α, pgc-1β, ucp1, ucp2, pparαa, pparαb, pparβ, pparγ, leptin and lpl. These results demonstrate that zbmp8a is involved in the regulation of lipid metabolism in zebrafish. Our finding provides the first data about the role of bmp8a in energy homeostasis in teleost.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene Reports - Volume 10, March 2018, Pages 33-41
Journal: Gene Reports - Volume 10, March 2018, Pages 33-41
نویسندگان
Shenjie Zhong, Ying Wang, Juan Li, Min Wang, Li Meng, Zengyu Ma, Shicui Zhang, Zhenhui Liu,