کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8646272 | 1570075 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Contribution of efflux systems to the detergent resistance, cytotoxicity, and biofilm formation of Vibrio vulnificus
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کلمات کلیدی
RNDCFUVibrio vulnificusqRT-PCRMICcDNA - cDNAComplementary DNA - DNA تکمیلیGene expression - بیان ژنTriton X-100 - تریتون X-100 Minimum inhibitory concentration - حداقل غلظت مهاریlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH Antimicrobial resistance - مقاومت ضد میکروبیDetergent - مواد شویندهBile salts - نمک صفاقیquantitative reverse transcription polymerase chain reaction - واکنش زنجیره ای پلی مراز رونویسی معکوسpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازVirulence - ویروسی شدنEfflux pump - پمپ Effluxcolony forming unit - کلنی واحد تشکیل
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Contribution of efflux systems to the detergent resistance, cytotoxicity, and biofilm formation of Vibrio vulnificus Contribution of efflux systems to the detergent resistance, cytotoxicity, and biofilm formation of Vibrio vulnificus](/preview/png/8646272.png)
چکیده انگلیسی
Vibrio vulnificus causes primary septicemia in immunocompromised patients following consumption of contaminated seafood. In order to cause infection, pathogenic bacteria require mechanisms to defend against the action of host-derived antimicrobials, and may accomplish this task through the expression of efflux pumps. Herein, we investigated the influence of bile salts on the antimicrobial resistance mechanisms in V. vulnificus, and the contribution of efflux pumps to the bacterium's detergent resistance and virulence characteristics. Bacterial cells treated with either bile salts or Triton X-100 developed enhanced detergent resistance relative to the untreated control, and also showed increased expression of genes encoding resistance-nodulation-division (RND) efflux pumps. Further investigation on V. vulnificus mutants deficient in RND efflux pump VexB revealed them to be more susceptible to nonionic detergents. In addition, an RND efflux pump triple mutant, ÎvexBHZ, exhibited increased cytotoxicity and enhanced biofilm formation. In summary, our data indicate that V. vulnificus RND efflux pump VexB plays a key role in the resistance to nonionic detergents. Furthermore, the expression levels of efflux pumps affect the antimicrobial resistance and are involved in modulating the biofilm formation and cytotoxicity of this pathogen.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene Reports - Volume 9, December 2017, Pages 115-122
Journal: Gene Reports - Volume 9, December 2017, Pages 115-122
نویسندگان
Chung-Cheng Lo, Pei-Ting Lin, Chuan Chiang-Ni, Kuan-Hua Lin, Shin-Luen Lee, Tzu-Fang Kuo, Horng-Ren Lo,