کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8920159 1643341 2013 17 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Defining synthetic surfaces for human pluripotent stem cell culture
ترجمه فارسی عنوان
تعریف سطوح مصنوعی برای کشت سلول های بنیادی پلوروپتوز انسانی
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Human pluripotent stem cells (hPSCs) are able to self-renew indefinitely and to differentiate into all adult cell types. hPSCs therefore show potential for application to drug screening, disease modelling and cellular therapies. In order to meet this potential, culture conditions must be developed that are consistent, defined, scalable, free of animal products and that facilitate stable self-renewal of hPSCs. Several culture surfaces have recently been reported to meet many of these criteria although none of them have been widely implemented by the stem cell community due to issues with validation, reliability and expense. Most hPSC culture surfaces have been derived from extracellular matrix proteins (ECMPs) and their cell adhesion molecule (CAM) binding motifs. Elucidating the CAM-mediated cell-surface interactions that are essential for the in vitro maintenance of pluripotency will facilitate the optimisation of hPSC culture surfaces. Reports indicate that hPSC cultures can be supported by cell-surface interactions through certain CAM subtypes but not by others. This review summarises the recent reports of defined surfaces for hPSC culture and focuses on the CAMs and ECMPs involved.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Regeneration - Volume 2, Issue 1, 2013, 2:7
نویسندگان
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