کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9034490 | 1562440 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Activation of CYP1A1 gene expression during primary culture of mouse hepatocytes
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کلمات کلیدی
DCFH-DACYP1A2XREARNTCYP1A1HspTCDDNF-1GAPDHSSCCyPAHR2,3,7,8-Tetrachlorodibenzo-p-dioxin - 2،3،7،8-تترا کلریدیبنزوپتوفان دیوکسینcDNA - cDNAComplementary DNA - DNA تکمیلیstandard saline citrate - استاندارد سدیم سیتراتOkadaic acid - اسید اوکادائیکSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدOxidative stress - تنش اکسیداتیوCytochrome P450 - سیتوکروم پی۴۵۰Anti-oxidant - ضد اکسید کنندهnuclear factor 1 - عامل هسته ای 1xenobiotic-responsive element - عنصر واکنش زنجبیلProtein kinase inhibitor - مهار کننده پروتئین کینازHeat shock protein - پروتئین شوک حرارتglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازaryl hydrocarbon receptor - گیرنده آرویل هیدروکربن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Activation of CYP1A1 gene expression during primary culture of mouse hepatocytes Activation of CYP1A1 gene expression during primary culture of mouse hepatocytes](/preview/png/9034490.png)
چکیده انگلیسی
Expression of CYP1A1 mRNA in mouse hepatocytes in primary culture was investigated. The expression was obvious on day 3 of culture without addition of any known ligands of the aryl hydrocarbon receptor and increased with culture period. Removal of insulin from and addition of hydrogen peroxide to the medium enhanced and suppressed the expression, respectively. The CYP1A1 mRNA expression was also enhanced in the presence of anti-oxidant, t-butylhydroquinone, in the medium. Several kinds of kinase inhibitors markedly increased the CYP1A1 mRNA expression. In contrast, the inhibitory expression was prolonged in the presence of okadaic acid, a potent inhibitor of serine/threonine phosphatase PP1 and PP2. These observations suggest that there might be a repressive pathway in the regulation of CYP1A1 mRNA expression and that the presently observed expression pathway differs at several points from those previously reported, such as ligand-activated aryl hydrocarbon receptor- or omeprazole-mediated expression. Modulation of CYP1A2 mRNA expression after exposing hepatocytes to agents affecting phosphorylation pathways differed from that of CYP1A1 mRNA. This implies that regulatory pathways for CYP1A1 and CYP1A2 expression may differ.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 216, Issues 2â3, 15 December 2005, Pages 224-231
Journal: Toxicology - Volume 216, Issues 2â3, 15 December 2005, Pages 224-231
نویسندگان
Hisako Tamaki, Tsutomu Sakuma, Yo-ichi Uchida, Atika Jaruchotikamol, Nobuo Nemoto,