کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9038473 | 1133837 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Organochlorine insecticides: impacts on human HepG2 cytochrome P4501A, 2B activities and glutathione levels
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کلمات کلیدی
OC, organochlorineGSSG, glutathione oxidizedDMEM, Dulbecco’s minimum essential mediumGSH, glutathione reduced - GSH، گلوتاتیون کاهش می یابدGST, glutathione S-transferase - GST، glutathione S-transferasePCBs, polychlorinated biphenyls - PCBs، bifenyls polychlorinatedPCDFs, polychlorinated dibenzofurans - PCDFs، dibenzofurans پلی کربناتROS, Reactive Oxygen Species - ROS، انواع اکسیژن واکنشیOxidative stress - تنش اکسیداتیوDMSO, dimethylsulfoxide - دیمتیل سولفواکسیدCYP, cytochrome P450 - سیتوکروم P450Cytochrome P450 - سیتوکروم پی۴۵۰PBS, phosphate buffered saline - محلول نمکِ فسفات با خاصیت بافریGR, glutathione reductase - گلوتاتیون ردوکتازGPX, glutathione peroxidase - گلوتاتیون پراکسیداز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Organochlorine insecticides: impacts on human HepG2 cytochrome P4501A, 2B activities and glutathione levels Organochlorine insecticides: impacts on human HepG2 cytochrome P4501A, 2B activities and glutathione levels](/preview/png/9038473.png)
چکیده انگلیسی
This study examined the effects of the organochlorine (OC) insecticides chlordane, o,pâ²-DDT, dieldrin, endosulfan, kepone, methoxychlor, and toxaphene on human HepG2 cytochrome P450 (1A-EROD and 2B-PROD) activities and glutathione (GSH) levels. Cells were exposed for 24Â h at high concentrations (1, 5 or 10Â mM) and for 48Â h at lower concentrations ranging from 0.01 to 1Â mM to evaluate dose responses. Our results show that after 48Â h all but dieldrin significantly induced both P4501A and 2B. P4502B responses were greater at all exposure concentrations and times. Mixed responses in GSH levels were observed. All OCs except dieldrin and MXC significantly depleted GSH after 24Â h. At 48Â h, chlordane, endosulfan and toxaphene significantly increased GSH at low levels and decreased GSH at high levels, while kepone and methoxychlor produced significant declines in GSH at all concentrations. These results support findings of OC insecticides inducing CYP1A, 2B in rats, with CYP2B responses more important. GSH levels declined when P4502B activity was significantly elevated and were significantly increased in the absence of significant P450 activity, suggesting that GSH levels influence the catalytic activity of the cytochrome P450s and the cytochrome P450s influence the cell's ability to regulate GSH.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 19, Issue 2, March 2005, Pages 261-273
Journal: Toxicology in Vitro - Volume 19, Issue 2, March 2005, Pages 261-273
نویسندگان
P.F. Dehn, S. Allen-Mocherie, J. Karek, A. Thenappan,