کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9038788 | 1133873 | 2005 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Culture of the primary corneal epithelium as a potential component of test batteries for eye irritancy testing
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کلمات کلیدی
FBS, foetal bovine serum - FBS، سرم جنین گاوGAPDH, glyceraldehyde-3-phosphate dehydrogenase - GAPDH، glyceraldehyde-3-phosphate dehydrogenaseHBsAg, hepatitis B Surface Antigen - HBsAg، آنتیژن سطح هپاتیت BIL-1β, interleukin-1β - IL-1β، اینترلوکین-1βMAC, membrane attack complex - MAC، پیچیده حمله غشاءMTT, 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide - MTT، 3- (4،5-Dimethylthiazol-2-yl) -2،5-Diphenyltetrazolium bromideRT-PCR, reverse transcriptase polymerase chain reaction - RT-PCR، واکنش زنجیره ای پلی مراز ترانس کریتاز معکوسinflammation - التهاب( توروم) ELISA, enzyme-linked immunosorbent assay - تست الیزاDMSO, dimethyl sulfoxide - دیمتیل سولفواکسیدEye irritation - سوزش چشمCytokines - سیتوکین هاCornea - قرنیهlipopolysaccharide - لیپوپلی ساکاریدComplement - متممprotectin - محافظPBS, phosphate-buffered saline - محلول نمک فسفات با خاصیت بافریLPS, lipopolysaccharide - مولکولهای لیپوپلی ساکاریدMops, 4-morpholinepropanesulfonic acid - مگس، 4-مورفولین پروپان سولفونیک اسیدHIV, human immunodeficiency virus - ویروس نقص ایمنی انسانیHCV, hepatitis C virus - ویروس هپاتیت سی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
One of the main goals for toxicologists working on the development of in vitro tests is to replace the animal-based eye irritation test. Inflammation is one of the mechanisms which have not been covered sufficiently by the existing in vitro ocular irritancy test systems. As there are major species differences between the human and rabbit eye inflammation mechanisms, the most relevant test system is the human eye itself. The current study focused on an evaluation of the practical availability of human corneal epithelial cells for routine eye irritancy testing. Human corneal epithelium cell cultures were used to assess the effects of lipopolysaccharide on IL-1β release. The findings indicated that cytokine release can be augmented by the presence of the complement system, which is normally found in tears. However, the corneal cells were found to be highly resistant to the complement system, which can be attributed to the very high expression of CD59, a powerful complement regulatory protein found in the corneal epithelium. It is estimated that discarded corneas from tissue banks could provide enough material for routine testing by this method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 19, Issue 7, October 2005, Pages 875-878
Journal: Toxicology in Vitro - Volume 19, Issue 7, October 2005, Pages 875-878
نویسندگان
D. Åladowski, I. Liberek, K. Lipski, T. Ozga, J. Olkowska-truchanowicz, J. Szaflik,