کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9573226 | 1503986 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lessons from crystals grown in the Advanced Protein Crystallisation Facility for conventional crystallisation applied to structural biology
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کلمات کلیدی
ApcFμgHYPPCDFFWHMISSESAADHVDAPDBCcdBEuropean Space Agency - آژانس فضایی اروپاAlcohol dehydrogenase - الکل دهیدروژنازInternational space station - ایستگاه فضایی بین المللیMicrogravity - ریزگرانش یا میکروگرانشGround - زمینProtein structure - ساختار پروتئینfull width at half maximum - عرض کامل در نیمی از حداکثرResolution limit - محدودیت قطعنامهhydroxyproline - هیدروکسی پرولینDiffusion - واپخش یا انتشار Molecular weight - وزن مولکولیatomic displacement parameter - پارامتر جابجایی اتمیProtein Data Bank - پروتئین بانک اطلاعاتیCrystal quality - کیفیت کریستال
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
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چکیده انگلیسی
The crystallographic quality of protein crystals that were grown in microgravity has been compared to that of crystals that were grown in parallel on earth gravity under otherwise identical conditions. A goal of this comparison was to assess if a more accurate 3D-structure can be derived from crystallographic analysis of the former crystals. Therefore, the properties of crystals prepared with the Advanced Protein Crystallisation Facility (APCF) on earth and in orbit during the last decade were evaluated. A statistical analysis reveals that about half of the crystals produced under microgravity had a superior X-ray diffraction limit with respect of terrestrial controls. Eleven protein structures could be determined at previously unachieved resolutions using crystals obtained in the APCF. Microgravity induced features of the most relevant structures are reported. A second goal of this study was to identify the cause of the crystal quality enhancement useful for structure determination. No correlations between the effect of microgravity and other system-dependent parameters, such as isoelectric point or crystal solvent content, were found except the reduced convection during the crystallisation process. Thus, crystal growth under diffusive regime appears to be the key parameter explaining the beneficial effect of microgravity on crystal quality. The mimicry of these effects on earth in gels or in capillary tubes is discussed and the practical consequences for structural biology highlighted.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 118, Issues 2â3, 1 December 2005, Pages 102-112
Journal: Biophysical Chemistry - Volume 118, Issues 2â3, 1 December 2005, Pages 102-112
نویسندگان
Alessandro Vergara, Bernard Lorber, Claude Sauter, Richard Giegé, Adriana Zagari,