کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9605533 | 44164 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Spectroscopic studies on binding of shikonin to human serum albumin
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کلمات کلیدی
HSAFourier transform IRhuman serum albumin - آلبومین سرم انسانیShikonin - تماشایFluorescence quenching - خنک شدن فلورسنتcircular dichroism - رنگ تابی دورانیCircular dichroism spectroscopy - طیف سنجی دی کرومFT-IR - طیف سنجی مادون قرمز تبدیل فوریهFourier transform infrared spectroscopy - طیف سنجی مادون قرمز تبدیل فوریه یا طیف سنجی FTIRMolecular modeling - مدل سازی مولکولی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
The binding properties on shikonin to human serum albumin (HSA) have been studied for the first time using fluorescence spectroscopy in combination with UV-visible absorbance spectroscopy, Fourier transform infrared (FT-IR) spectroscopy and circular dichroism (CD) spectroscopy. The results of spectroscopic measurements suggested that the hydrophobic interaction is the predominant intermolecular force stabilizing the complex, which is in good agreement with the results of molecule modeling study. And the enthalpy change ÎH° and the entropy change ÎS° were calculated to be â13.86 kJ molâ1 and 51.16 J molâ1 Kâ1 according to the Vant'Hoff equation. The fluorescence quenching mechanism and the number of binding site (n â 1) were also obtained from fluorescence titration data. The efficiency of Förster energy transfer provided a distance of 2.12 nm between tryophan and shikonin binding site. The alterations of protein secondary structure in the presence of shikonin in aqueous solution were quantitatively calculated from FT-IR and CD spectroscopy with reductions of α helices content about 2.8-5.4% and with increases of β structures about 2.4%. In addition, the effect of common ions on the binding constants of shikonin-HSA complexes was also discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 174, Issue 1, 1 August 2005, Pages 53-61
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 174, Issue 1, 1 August 2005, Pages 53-61
نویسندگان
Wenying He, Ying Li, Jianniao Tian, Huanxiang Liu, Zhide Hu, Xingguo Chen,