کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9884693 | 1537059 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The transient complex of poplar plastocyanin with cytochrome f: effects of ionic strength and pH
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کلمات کلیدی
Cytochrome ftransient complexNOESYTOCSYPMSFIPTGHSQCTSPnuclear magnetic resonance - رزونانس مغناطیسی هستهایisopropylthio-β-d-galactoside - isopropylthio-β-d galactosideNuclear Overhauser Effect SpectroscopY - Spectroscopy اثر Overhauser هسته ایHeteronuclear Single-Quantum Coherence Spectroscopy - اسپکتروسکوپ تک هسته ای تک کوانتومی تک هسته ایelectron transfer - انتقال الکترونNMR - تشدید مغناطیسی هستهای PCs - رایانه های شخصیNMR spectroscopy - طیف سنجی رزونانس مغناطیسی هستهTotal correlation spectroscopy - طیف سنجی مجموع همبستگیPhotosynthesis - فتوسنتزProtein complex - پروتئین پیچیدهPlastocyanin - پلاستوسیانین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The transient complex of poplar plastocyanin with cytochrome f: effects of ionic strength and pH The transient complex of poplar plastocyanin with cytochrome f: effects of ionic strength and pH](/preview/png/9884693.png)
چکیده انگلیسی
The orientation of poplar plastocyanin in the complex with turnip cytochrome f has been determined by rigid-body calculations using restraints from paramagnetic NMR measurements. The results show that poplar plastocyanin interacts with cytochrome f with the hydrophobic patch of plastocyanin close to the heme region on cytochrome f and via electrostatic interactions between the charged patches on both proteins. Plastocyanin is tilted relative to the orientation reported for spinach plastocyanin, resulting in a longer distance between iron and copper (13.9 Ã
). With increasing ionic strength, from 0.01 to 0.11 M, all observed chemical-shift changes decrease uniformly, supporting the idea that electrostatic forces contribute to complex formation. There is no indication for a rearrangement of the transient complex in this ionic strength range, contrary to what had been proposed earlier on the basis of kinetic data. By decreasing the pH from pH 7.7 to pH 5.5, the complex is destabilized. This may be attributed to the protonation of the conserved acidic patches or the copper ligand His87 in poplar plastocyanin, which are shown to have similar pKa values. The results are interpreted in a two-step model for complex formation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1707, Issues 2â3, AprilâMay 2005, Pages 179-188
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1707, Issues 2â3, AprilâMay 2005, Pages 179-188
نویسندگان
Christian Lange, Tobias Cornvik, Irene DÃaz-Moreno, Marcellus Ubbink,