کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9886615 | 1537840 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization of the lipopolysaccharide/platelet activating factor- and zymosan-induced pathways leading to prostaglandin production in P388D1 macrophages
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کلمات کلیدی
LPSmPGESH-PGDSPIP2sPLA2PLA2CHXActDRP-HPLCCOXGAPDHTLCMAPK - MAPKcyclooxygenase - آنزیم سیکلواکسیژنازphospholipase A2 - آنزیم فسفولیپاز A2 Arachidonic acid - اسید آراشیدونیکhydroxyeicosatetraenoic acid - اسید هیدروکسی اسیستاترونیکactinomycin D - اکتینومایسین Dthromboxane - ترومبوکسیانZymosan - زیموسانcycloheximide - سیکلوهایسیمیدPlatelet-activating factor - فاکتور فعال کننده پلاکتphosphatidylinositol 4,5-bisphosphate - فسفاتیدیلینوزیتول 4،5-بیسفسفاتlipopolysaccharide - لیپوپلی ساکاریدMacrophage - ماکروفاژ PAF - نیروی هوایی پاکستانHETE - هفتهmitogen activated protein kinase - پروتئین کیناز فعال Mitogen فعال استprostaglandin - پروستاگلاندینهاthin layer chromatography - کروماتوگرافی لایه نازکReverse-phase high performance liquid chromatography - کروماتوگرافی مایع با کارایی معکوس فازglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
P388D1 cells release free arachidonic acid (AA) and prostaglandin E2 (PGE2) upon stimulation with platelet-activating factor (PAF) and zymosan. The response to PAF is dependent on priming of the cells with bacterial lipopolysaccharide (LPS). In the LPS/PAF pathway, both AA and PGE2 release are dependent on transcription and translation, whereas in the zymosan pathway the release of these compounds appears to be largely independent of these processes. Using quantitative real-time PCR, we analyzed the expression of mRNAs that encode proteins potentially responsible for the dependency of the LPS/PAF pathway on gene expression. These include all the phospholipases A2 (PLA2) that we detected in P388D1 cells, cyclooxygenases (COX), COX-1 and COX-2, the membrane-associated prostaglandin E synthase-1 (mPGES-1), the lipocalin-type prostaglandin D2 synthase (PGDS), hematopoietic PGDS and the subunit Gαi2 of heterotrimeric G-proteins. None of the mRNAs encoding PLA2s, PGDSs, or Gαi2 are substantially altered during LPS priming. However, cyclooxygenase-2 is up-regulated during LPS priming and after stimulation of the cells with zymosan. A modest but significant increase of mPGES-1 mRNA was also detected upon stimulation with zymosan. Thus, the dependency of the LPS/PAF-induced PGE2 production on gene expression can be attributed to the production of cyclooxygenase-2. The dependency of AA release on gene expression is not due to altered expression of any of the PLA2s. We suggest that an accessory regulatory protein affecting the release of AA must be responsible. Using HPLC we separated lipids that are secreted upon stimulation with LPS/PAF and zymosan and found that in both pathways PGD2 is the dominant prostaglandin produced and also detected PGE2, PGF2α and AA besides several unidentified compounds.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1687, Issues 1â3, 21 February 2005, Pages 64-75
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1687, Issues 1â3, 21 February 2005, Pages 64-75
نویسندگان
Ralph H. Schaloske, Jarrod W. Provins, Ursula A. Kessen, Edward A. Dennis,