Galactonolactone oxidoreductase from Trypanosoma cruzi employs a FAD cofactor for the synthesis of vitamin C

► Trypanosoma cruzi galactonolactone oxidoreductase was refolded in reverse micelles. ► Refolded TcGAL is active with L-galactono-1,4-lactone and D-arabinono-1,4-lactone. ► TcGAL employs FAD as cofactor for the synthesis of vitamin C. ► TcGAL can use molecular oxygen as electron acceptor.