کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10541846 | 963435 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inhibitory effects of flavonoid glycosides isolated from the peel of Japanese persimmon (Diospyros kaki Fuyu) on antigen-stimulated degranulation in rat basophilic leukaemia RBL-2H3 cells
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کلمات کلیدی
IgEPLCDNPSRCERKHRPEGCGPKCHYPDPPHFcεRIRUTISQCM-H2DCF-DADPI(−)-epigallocatechin gallate - (-) - epigallocatechin گالات1,1-diphenyl-2-picrylhydrazyl - 1،1-دیفنیل-2-پریکیل هیدرازیلBSA - BSAROS - ROSSAPK/JNK - SAPK / JNKbovine serum albumin - آلبومین سرم گاوIsoquercitrin - اسکوکریتروینImmunoglobulin E - ایمونوگلوبولین EQue - اینSyk - بیمارdiphenyleneiodonium chloride - دی فینیلنیدونیم کلریدdinitrophenol - دینیتروفنلspleen tyrosine kinase - طرز تیروزین کینازphospholipase C - فسفولیپاز CFlavonoid glycoside - فلاونوئید گلیکوزیدDegranulation - قرمزی چشمRutin - معمولیHyperin - هیپرینAntigen - پادگِن یا آنتیژنHorseradish peroxidase - پراکسیداز هوررادیشProtein kinase C - پروتئین کیناز سیmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenQuercetin - کوئرستینextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولیReactive oxygen species - گونههای فعال اکسیژنHigh affinity IgE receptor - گیرنده IgE باکتری بالا
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Inhibitory effects of flavonoid glycosides isolated from the peel of Japanese persimmon (Diospyros kaki Fuyu) on antigen-stimulated degranulation in rat basophilic leukaemia RBL-2H3 cells Inhibitory effects of flavonoid glycosides isolated from the peel of Japanese persimmon (Diospyros kaki Fuyu) on antigen-stimulated degranulation in rat basophilic leukaemia RBL-2H3 cells](/preview/png/10541846.png)
چکیده انگلیسی
We found that two distinct flavonoid glycosides isolated from the peel of Japanese persimmon (Diospyros kaki Fuyu), isoquercitrin (Isq) and hyperin (Hyp), are capable of inhibiting antigen-stimulated degranulation in rat basophilic leukaemia RBL-2H3 cells. In order to elucidate the underlying mechanisms, we examined effects of Isq and Hyp on cellular responses induced by antigen stimulation. Treatment with both Isq and Hyp markedly inhibited antigen-stimulated elevation of intracellular free Ca2+ concentration and reactive oxygen species (ROS). Isq and Hyp did not affect NADPH oxidase (NOX) activity, but they possessed DPPH radical-scavenging activity similar to that of epigallocatechin gallate, a potent anti-oxidant, Finally, Isq and Hyp showed little or no effects on Ag-stimulated Syk activation or phosphorylation of signalling molecules. These results indicate that inhibition of antigen-stimulated degranulation by Isq and Hyp is mainly due to suppression of intracellular Ca2+ elevation, which is caused by direct scavenging of ROS that are generated by NOX. Our findings suggest that Isq and Hyp, isolated from the peel of persimmon, would be beneficial for alleviating symptoms of type I allergy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 126, Issue 1, 1 May 2011, Pages 289-294
Journal: Food Chemistry - Volume 126, Issue 1, 1 May 2011, Pages 289-294
نویسندگان
Tomohiro Itoh, Kenji Ohguchi, Chizuru Nakajima, Masayoshi Oyama, Munekazu Iinuma, Yoshinori Nozawa, Yukihiro Akao, Masafumi Ito,