کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10548295 | 965436 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection of processed genetically modified food using CIM monolithic columns for DNA isolation
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کلمات کلیدی
Genetically modified organisms - ارگانیسم های اصلاح شده ژنتیکیDNA isolation - جداسازی DNAMaize - ذرت یا جواریConvective interaction media - رسانه متقابل همجوشیMonolithic columns - ستونهای یکپارچهProcessed foods - غذاهای فراوری شدهGenetically modified food - غذای اصلاح شده ژنتیکیpolymerase chain reaction - واکنش زنجیره ای پلیمرازreal-time polymerase chain reaction - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیFood control - کنترل غذا
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The availability of sufficient quantities of DNA of adequate quality is crucial in polymerase chain reaction (PCR)-based methods for genetically modified food detection. In this work, the suitability of anion-exchange CIM (Convective Interaction Media; BIA Separations, Ljubljana, Slovenia) monolithic columns for isolation of DNA from food was studied. Maize and its derivates corn meal and thermally pre-treated corn meal were chosen as model food. Two commercially available CIM disk columns were tested: DEAE (diethylaminoethyl) and QA (quaternary amine). Preliminary separations were performed with standard solution of salmon DNA at different pH values and different NaCl concentrations in mobile phase. DEAE groups and pH 8 were chosen for further isolations of DNA from a complex matrix-food extract. The quality and quantity of isolated DNA were tested on agarose gel electrophoresis, with UV-scanning spectrophotometry, and by amplification with real-time PCR. DNA isolated in this way was of suitable quality for further PCR analyses. The described method is also applicable for DNA isolation from processed foods with decreased DNA content. Furthermore, it is more effective and less time-consuming in comparison with the existing proposed methods for isolation of DNA from plant-derived foods.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1065, Issue 1, 11 February 2005, Pages 107-113
Journal: Journal of Chromatography A - Volume 1065, Issue 1, 11 February 2005, Pages 107-113
نویسندگان
Sergej Jerman, AleÅ¡ Podgornik, Katarina Cankar, Neža Äadež, Mihaela Skrt, Jana Žel, Peter Raspor,