کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10552727 | 967589 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A simple liquid chromatography coupled to quadrupole time of flight mass spectrometry method for macrolide determination in tilapia fillets
ترجمه فارسی عنوان
یک کروماتوگرافی مایع ساده همراه با زمان چهار بعدی از طیف سنجی جرمی پرواز برای تعیین ماکرولیدها در فیله تیلایپیا
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کلمات کلیدی
Aquaculture - آبزی پروریFood contaminants - آلاینده های مواد غذاییFood safety - ایمنی غذاییVeterinary drug residues - باقی مانده داروهای دامپزشکیAntimicrobial residues - باقی مانده های ضد میکروبیFood analysis - تجزیه و تحلیل مواد غذاییTilapia - تیلاپیاMass spectrometry - طیف سنجی جرمیmacrolides - ماکرولیدهاliquid chromatography - کروماتوگرافی مایع
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A method for the identification and quantification of macrolides (erythromycin, josamycin, tilmicosin, tylosin, spiramycin and neospiramycin) in tilapia fillets by liquid chromatography coupled to quadrupole time of flight mass (LC-QToF) spectrometry is presented. Sample preparation was quite simple and low cost: proteins were precipitated and the analytes were extracted with ethanol, extract was defatted with hexane and concentrated by solvent evaporation. The matrix effect was statistically demonstrated during method validation, in which matrix-matched calibration was applied. Matrix effect mechanism was clarified thanks to the capability of QToF mass spectrometer of generating full scan spectra with accurate mass measurement. The limits of quantification were at least 45% lower than the maximum residue limits. The method was able to identify the studied macrolides with relative m/z errors lower than 2.5 ppm and to monitor two fragment ions per analyte, which is in accordance with the European Community recommendations for the analysis of contaminants in foods. Samples from the retail market of São Paulo State, Brazil, were analyzed by the developed method and none of them presented positive results for the macrolides studied.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Food Composition and Analysis - Volume 34, Issue 2, June 2014, Pages 153-162
Journal: Journal of Food Composition and Analysis - Volume 34, Issue 2, June 2014, Pages 153-162
نویسندگان
Marcela Sismotto, Jonas A.R. Paschoal, Juliana A. Teles, Renata A.E. de Rezende, Felix G.R. Reyes,