کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10552872 | 967620 | 2012 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of lipophilic compounds in genetically modified rice using gas chromatography-time-of-flight mass spectrometry
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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![عکس صفحه اول مقاله: Determination of lipophilic compounds in genetically modified rice using gas chromatography-time-of-flight mass spectrometry Determination of lipophilic compounds in genetically modified rice using gas chromatography-time-of-flight mass spectrometry](/preview/png/10552872.png)
چکیده انگلیسی
An accurate and sensitive method for determining 14 lipophilic compounds, including tocopherols, tocotrienols, sterols and policosanols from rice (Oryza sativa L.) grain was developed using gas chromatography (GC) coupled to time-of-flight mass spectrometry (TOFMS). The method is fast, requiring only 13 min for the GC-TOFMS run. During the validation procedure, the method proved to be sufficiently precise and accurate with respect to the degree of endogenous biological variability found in rice samples. The profiles of the 14 lipophilic compounds of pigmented rice, as well as genetically modified (GM) rice to improve β-carotene, were subjected to principal component analysis (PCA) to evaluate the differences among cultivars. PCA could fully distinguish pigmented rice from non-pigmented rice. However, GM rice could not be separated from its non-transgenic counterpart (Nakdongbyeo). This protocol provides a rapid and feasible method for monitoring the bioactive compounds of rice.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Food Composition and Analysis - Volume 25, Issue 1, February 2012, Pages 31-38
Journal: Journal of Food Composition and Analysis - Volume 25, Issue 1, February 2012, Pages 31-38
نویسندگان
Jae Kwang Kim, Sun-Hwa Ha, Soo-Yun Park, Si Myung Lee, Hyo Jin Kim, Sun Hyung Lim, Seok-Cheol Suh, Dong Hern Kim, Hyun Suk Cho,