کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10553662 | 967888 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dunaliella salina extract effect on diabetic rats: Metabolic fingerprinting and target metabolite analysis
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کلمات کلیدی
PLS-DAGSHGSSGHFIPhexafluoroisopropanolBGEPCA - PCAEDTA - اتیلن دی آمین تترا استیک اسید Capillary electrophoresis - الکتروفورزمویرگیbackground electrolyte - الکترولیت پس زمینهPrincipal component analysis - تحلیل مولفههای اصلی یا PCAanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of variancelatent variable - متغیر غیرواقعیreduced glutathione - کاهش گلوتاتیونoxidized glutathione - گلوتاتیون اکسید شده
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Five doses of D. salina extract with in vitro antioxidant properties were intragastrically administrated to adult male streptozotocin (STZ) diabetic rats. Urine fingerprints of control and diabetic rats, both with and without treatment, were obtained by capillary electrophoresis with two different modes (normal polarity and MEKC and reverse polarity and CZE). When the profiles were submitted together to pattern recognition techniques they showed the effects of D. salina extract on this acute and short-term treatment animal model in a rapid, simple and cost-effective way without identifying a single marker. In order to have a further biochemical knowledge of the effect, after treatment, rats were sacrificed and blood and liver glutathione, as well as plasma glucose, triglycerides, cholesterol, total protein, urea, acetoacetate, 3-hydroxybutyrate, lactate, pyruvate and urate, TBARS and urine 8-isoprostane were analysed. Vitamin E in plasma and liver was also measured. Twenty-seven parameters were individually assessed, and both univariate statistics (mean comparison after 1W-ANOVA) and multivariate data analysis were performed. D. salina extract induced changes showed up by the multivariate analysis. Results of the treatment from most of the parameters can be considered beneficial for diabetic animals; although an increase in hyperglycemia and 8-isoprostane excretion when STZ treated animals received the extract was observed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 49, Issue 3, 5 April 2009, Pages 786-792
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 49, Issue 3, 5 April 2009, Pages 786-792
نویسندگان
Francisco J. Ruperez, Diana Garcia-Martinez, Beatriz Baena, Nuria Maeso, Maria Vallejo, Santiago Angulo, Antonia Garcia, Elena Ibañez, Francisco J. Señorans, Alejandro Cifuentes, Coral Barbas,