کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10559447 | 969627 | 2011 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phospholipidomic identification of potential plasma biomarkers associated with type 2 diabetes mellitus and diabetic nephropathy
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کلمات کلیدی
PLSVIPTCMLPCPKCPLS-DAPLA2LOQT2DMRSDESRDESIphospholipaseA2PCA - PCAsphingomyelin - اسفنگومیلینrelative standard deviation - انحراف استاندارد نسبیPotential biomarkers - بیومارکرهای بالقوهPartial least squares discriminant analysis - تجزیه و تحلیل خرده مقیاس حداقل مربعات جزئیPrincipal component analysis - تحلیل مولفههای اصلی یا PCAanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceMultivariate analysis - تحلیل چندمتغیرهType 2 diabetes mellitus - دیابت نوع دوtraditional Chinese medicine - طب سنتی چینیphosphatidylinositol - فسفاتیدیل اینوزیتولphosphatidylcholine - فسفاتیدیل کولینphosphatidylglycerol - فسفاتیدیل گلیسرولphosphatidylethanolamine - فسفاتیدیلتانولامینPhosphatidylserine - فسفاتیدیلسرینPhospholipids - فسفولیپیدLod - لودLysophosphatidylcholine - لیزوفسفاتیدیل کولینMVA - مالیات بر ارزش افزودهlimit of detection - محدودیت تشخیصlimit of quantitation - محدودیت مقدارend stage renal disease - مرحله نهایی بیماری کلیویSorbitol pathway - مسیر سوربیتولMyo-inositol - میو-آنزیتولDiabetic nephropathy - نفروپاتی دیابتیProtein kinase C - پروتئین کیناز سیquality control - کنترل کیفیتelectrospray ionization - یونیزاسیون الکترو اسپری
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Type 2 diabetes mellitus (T2DM) and its attendant complications, such as diabetic nephropathy (DN), impose a significant societal and economic burden. The investigation of discovering potential biomarkers for T2DM and DN will facilitate the prediction and prevention of diabetes. Phospholipids (PLs) and their metabolisms are closely allied to nosogenesis and aggravation of T2DM and DN. The aim of this study is to characterize the human plasma phospholipids in T2DM and DN to identify potential biomarkers of T2DM and DN. Normal phase liquid chromatography coupled with time of flight mass spectrometry (NPLC-TOF/MS) was applied to the plasma phospholipids metabolic profiling of T2DM and DN. The plasma samples from control (n = 30), T2DM subjects (n = 30), and DN subjects (n = 52) were collected and analyzed. The significant difference in metabolic profiling was observed between healthy control group and DM group as well as between control group and DN group by the help of partial least squares discriminant analysis (PLS-DA). PLS-DA and one-way analysis of variance (ANOVA) were successfully used to screen out potential biomarkers from complex mass spectrometry data. The identification of molecular components of potential biomarkers was performed on Ion trap-MS/MS. An external standard method was applied to quantitative analysis of potential biomarkers. As a result, 18 compounds in 7 PL classes with significant regulation in patients compared with healthy controls were regarded as potential biomarkers for T2DM or DN. Among them, 3 DM-specific biomarkers, 8 DN-specific biomarkers and 7 common biomarkers to DM and DN were identified. Ultimately, 2 novel biomarkers, i.e., PI C18:0/22:6 and SM dC18:0/20:2, can be used to discriminate healthy individuals, T2DM cases and DN cases from each other group.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 85, Issue 4, 30 September 2011, Pages 1711-1720
Journal: Talanta - Volume 85, Issue 4, 30 September 2011, Pages 1711-1720
نویسندگان
Chao Zhu, Qiong-lin Liang, Ping Hu, Yi-ming Wang, Guo-an Luo,