کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10738837 | 1046836 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of Nrf2 activation and heme oxygenase-1 expression in NIH3T3 cells exposed to aqueous extracts of cigarette smoke
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کلمات کلیدی
AP-1EMSAγ-glutamyl-cysteine synthetaseMaf recognition elementTPA-response elementγGCSHeat-shock factor-1HSF-1GCLCNQO1HMOX1Heme oxygenase-1NF-κBFCSStreRT qPCRNrf2HO-1DMEMCREc/ebpβ - c / ebpβDulbecco's modified Eagle Medium - Eagle Medium اصلاح شده DulbeccoMTT - MTTNAD(P)H:quinone oxidoreductase - NAD (P) H: کینون اکسیدوردوکتازRNA interference - RNA تداخل کنندهRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهelectrophoretic mobility-shift assay - تحرک انتقال الکتریکی - تحرک تغییرOxidative stress - تنش اکسیداتیوCigarette smoke - دود سیگارFree radical - رادیکالهای آزاد fetal calf serum - سرم گوساله جنینTRE - سهNF-E2-related factor 2 - عامل NF-E2 2cAMP-response element - عنصر پاسخ cAMPantioxidant-responsive element - عنصر پاسخ دهنده آنتی اکسیدانnuclear factor κB - فاکتور هسته ای κBglutamate-cysteine ligase, catalytic subunit - لیگاز گلوتامات سیستئین، واحد کاتالیزوریmare - مادیانPhase II - مرحله دومwild-type - نوع وحشیARE - هستندActivated protein 1 - پروتئین فعال 1
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Characterization of Nrf2 activation and heme oxygenase-1 expression in NIH3T3 cells exposed to aqueous extracts of cigarette smoke Characterization of Nrf2 activation and heme oxygenase-1 expression in NIH3T3 cells exposed to aqueous extracts of cigarette smoke](/preview/png/10738837.png)
چکیده انگلیسی
Cigarette smoke (CS) is a complex chemical mixture estimated to be composed of up to 5000 different chemicals, many of which are prooxidant. Here we show that, at least in vitro, the cellular response designed to combat oxidative stress resulting from CS exposure is primarily controlled by the transcription factor Nrf2, a principal inducer of antioxidant and phase II-related genes. The prominent role of Nrf2 in the cellular response to CS is substantiated by the following observations: In NIH3T3 cells exposed to aqueous extracts of CS (i) Nrf2 is strongly stabilized and becomes detectable in nuclear extracts. (ii) Nuclear localization of Nrf2 coincides with increased DNA binding of a putative Nrf2/MafK heterodimer to its cognate cis-regulatory site, i.e., the antioxidant-responsive element (ARE). (iii) Studies on the regulatory elements of the oxidative stress-inducible gene heme oxygenase-1 (hmox1) using various hmox1 promoter/luciferase reporter constructs revealed that the strong CS-dependent expression of this gene is primarily governed by the distal enhancers 1 (“E1”) and 2 (“E2”), which both contain three canonical ARE-like stress-responsive elements (StREs). Notably, depletion of Nrf2 levels caused by RNA interference significantly compromised CS-induced hmox1 promoter activation, based on the distinct Nrf2 sensitivity exhibited by E1 and E2. Finally, (iv) siRNA-dependent knock-down of Nrf2 completely abrogated CS-induced expression of phase II-related genes. Taken together, these results confirm the outstanding role of Nrf2 both in sensing (oxidant) stress and in orchestrating an efficient transcriptional response aimed at resolving the stressing conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 39, Issue 11, 1 December 2005, Pages 1438-1448
Journal: Free Radical Biology and Medicine - Volume 39, Issue 11, 1 December 2005, Pages 1438-1448
نویسندگان
Constanze Knörr-Wittmann, Arnd Hengstermann, Stephan Gebel, Jawed Alam, Thomas Müller,