کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10757098 | 1050392 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Reprogramming of enteroendocrine K cells to pancreatic β-cells through the combined expression of Nkx6.1 and Neurogenin3, and reaggregation in suspension culture
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
DAPTKrebs Ringer bufferNgn3Pdx1GLUT2GIPGLP-1KRbDAPIGFPAdVDMEMITS4′,6′-diamidino-2-phenylindole - 4 '، 6'-diamidino-2-phenylindoleMOI - MEDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoN-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester - N- [N- (3،5-difluorophenacetyl) -L-alanyl] -S-phenylglycine t-butyl esterβ-Cells - β-سلول هاAdenovirus - آدنوویروسReprogramming - برنامه ریزی مجددDifferentiation - تفکیکIslet - جزیرهenteroendocrine cells - سلولهای enteroendocrineNeurogenin 3 - نوروژنین 3green fluorescent protein - پروتئین فلورسنت سبزprohormone convertase - پروژورمون تبدیلglucagon-like peptide-1 - پپتید 1-گلوکاگون-مانندIslet transplantation - پیوند جزیرهmultiplicity of infection - چندین عفونتglucose transporter 2 - گلوکز 2glucose-dependent insulinotropic polypeptide - گلیکول وابسته به پلی پپتید انسولینوتروپیک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Recent studies have demonstrated that adult cells such as pancreatic exocrine cells can be converted to pancreatic β-cells in a process called cell reprogramming. Enteroendocrine cells and β-cells share similar pathways of differentiation during embryonic development. Notably, enteroendocrine K cells express many of the key proteins found in β-cells. Thus, K cells could be reprogrammed to β-cells under certain conditions. However, there is no clear evidence on whether these cells convert to β-cells. K cells were selected from STC-1 cells, an enteroendocrine cell line expressing multiple hormones. K cells were found to express many genes of transcription factors crucial for islet development and differentiation except for Nkx6.1 and Neurogenin3. A K cell clone stably expressing Nkx6.1 (Nkx6.1+-K cells) was established. Induction of Neurogenin3 expression in Nkx6.1+-K cells, by either treatment with a γ-secretase inhibitor or infection with a recombinant adenovirus expressing Neurogenin3, led to a significant increase in Insulin1 mRNA expression. After infection with the adenovirus expressing Neurogenin3 and reaggregation in suspension culture, about 50% of Nkx6.1+-K cells expressed insulin as determined by immunostaining. The intracellular insulin content was increased markedly. Electron microscopy revealed the presence of insulin granules. However, glucose-stimulated insulin secretion was defective, and there was no glucose lowering effect after transplantation of these cells in diabetic mice. In conclusion, we demonstrated that K cells could be reprogrammed partially to β-cells through the combined expression of Nkx6.1 and Neurogenin3, and reaggregation in suspension culture.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 443, Issue 3, 17 January 2014, Pages 1021-1027
Journal: Biochemical and Biophysical Research Communications - Volume 443, Issue 3, 17 January 2014, Pages 1021-1027
نویسندگان
Esder Lee, Gyeong Ryul Ryu, Sung-Dae Moon, Seung-Hyun Ko, Yu-Bae Ahn, Ki-Ho Song,