کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10904144 | 1086562 | 2014 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Autophagy inhibition induces podocyte apoptosis by activating the pro-apoptotic pathway of endoplasmic reticulum stress
ترجمه فارسی عنوان
مهار واکنش بدن باعث آپوپتوز پودوسیت توسط فعال سازی پروپوپوپتوزیس تنش ریشه ای اندوپلاسمی می شود
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کلمات کلیدی
STZ3-MATBG3-methyladenine - 3-متیل آدنینSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNAAutophagy - اتوفاژیEndoplasmic reticulum stress - استرس شبکه آندوپلاسمیstreptozotocin - استرپتوزوتوسینCHOP - تکه کردنApoptosis - خزان یاختهایendoplasmic reticulum - شبکه آندوپلاسمی CCAAT-enhancer-binding protein homologous protein - پروتئین همولوگ زدن پروتئین پروتئین تقویت کننده CCAATPodocyte - پودوسیت
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
چکیده انگلیسی
Podocyte apoptosis is a major factor inducing podocyte depletion that predicts the progressive course of glomerulosclerosis. However, the molecular mechanisms underlying podocyte apoptosis are still not well understood. Autophagy is a lysosomal degradation system involving the degradation and recycling of obsolete, damaged, or harmful cytoplasmic materials and organelles. Recent advances in the understanding of the molecular processes contributing to autophagy have provided insight into the relationship between autophagy and apoptosis. However, their cross-talk remains largely obscure until now. Here, we found that podocytes both in vivo and in vitro always exhibited high basal levels of autophagy, whereas autophagy inhibition could induce podocyte apoptosis, suggesting the pro-survival role of autophagy in podocytes. Besides, we found that autophagy inhibition by 3-methyladenine (3-MA) could induce the activation of endoplasmic reticulum stress even without any external stimulations, whereas knockdown of CHOP could effectively improve podocyte apoptosis and down-regulated expression of slit-diaphragm proteins induced by autophagy inhibition. Collectively, this study demonstrated that autophagy might act as a crucial regulatory mechanism of apoptotic cell death by modulating the balance between the pro-survival pathway and the pro-apoptotic pathway of endoplasmic reticulum stress, which might provide a novel mechanistic insight into the interface between autophagy and apoptosis in the progression of podocyte injury.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 322, Issue 2, 1 April 2014, Pages 290-301
Journal: Experimental Cell Research - Volume 322, Issue 2, 1 April 2014, Pages 290-301
نویسندگان
Li Fang, Xiurong Li, Yuan Luo, Weichun He, Chunsun Dai, Junwei Yang,