کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11025654 | 1666540 | 2019 | 39 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The protective effects of DL-Selenomethionine against T-2/HT-2 toxins-induced cytotoxicity and oxidative stress in broiler hepatocytes
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کلمات کلیدی
FCSNACGSTGSHHT-2T-2CATDCFH-DAMDADMEM2′,7′-dichlorofluorescein diacetate - 2 '، 7'-dichlorofluorescein diacetateGSH-Px - GSH-PXHepatocytes - hepatocytesHT-2 toxin - HT-2 توکسینDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoMTT - MTTN-acetyl-cysteine - N-استیل سیستئینROS - ROSProtective effects - اثرات محافظتیThiazolyl blue tetrazolium bromide - تترازولیم برومید تیزولیل آبیOxidative stress - تنش اکسیداتیوT-2 toxin - توکسین T-2SOD - سدfetal calf serum - سرم گوساله جنینSelenium - سلنیومCytotoxicity - سمیت سلولیSuperoxide dismutase - سوکسوکس دیسموتازLDH - لاکتات دهیدروژناز به صورت مختصر شده LDH malondialdehyde - مالون دی آلدهیدCatalase - کاتالازGlutathione - گلوتاتیونglutathione S-transferase - گلوتاتیون S-ترانسفرازglutathione peroxidase - گلوتاتیون پراکسیداز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
T-2 and HT-2 toxins can cause cytotoxicity and oxidative stress in animals, while DL-Selenomethionine plays an important role in preventing oxidative stress and improving cell viability. However, the role of DL-Selenomethionine in T-2/HT-2 toxins-induced cell damage is still unknown. In this study, we investigated whether DL-Selenomethionine plays a protective role against T-2/HT-2-induced cytotoxicity and oxidative stress in primary hepatocytes. Our results demonstrated that T-2/HT-2 toxins-exposed broiler hepatocytes exhibited significantly decreased cell viability and intracellular glutathione (GSH) concentration while increased Lacate dehydrogenase (LDH) leakage, intracellular reactive oxygen species (ROS), glutathione peroxidase (GSH-PX), malondialdehyde (MDA) and catalase (CAT) levels, as well as elevated expression levels of genes related to oxidative stress, in a toxin dose-dependent manner (Pâ¯<â¯0.05). However, the application of DL-Selenomethionine into T-2/HT-2 treated hepatocytes effectively alleviated the adverse effects of T-2/HT-2, as demonstrated by increased cell viability, decreased LDH leakage, declined intracellular ROS and MDA levels, increased expression of oxidative stress-related genes, as well as accordingly enhanced activities of GSH, GSH-PX, SOD and CAT as compared to the control groups (Pâ¯<â¯0.05). Therefore, our in vitro data demonstrate that DL-Selenomethionine can function as an effectively protective agent against T-2/HT-2-induced cytotoxicity and oxidative stress.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 54, February 2019, Pages 137-146
Journal: Toxicology in Vitro - Volume 54, February 2019, Pages 137-146
نویسندگان
Lingchen Yang, Di Tu, Naidong Wang, Zhibang Deng, Yang Zhan, Wei Liu, Yi Hu, Tanbin Liu, Lei Tan, Yalan Li, Shiyin Guo, Aibing Wang,