کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1218861 | 1494510 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection of 3-N-oxalyl-l-2,3-diaminopropanoic acid in thermally processed foods by reverse phase high performance liquid chromatography
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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![عکس صفحه اول مقاله: Detection of 3-N-oxalyl-l-2,3-diaminopropanoic acid in thermally processed foods by reverse phase high performance liquid chromatography Detection of 3-N-oxalyl-l-2,3-diaminopropanoic acid in thermally processed foods by reverse phase high performance liquid chromatography](/preview/png/1218861.png)
چکیده انگلیسی
Grasspea (Lathyrus sativus), because of its low cost and ease of cultivation, is often an adulterant of other legumes. The seeds-albeit rich in protein (â¼20-32% of dry seed weight)-contain a neurotoxin, 3-N-oxalyl-l-2,3-diaminopropanoic acid (β-ODAP). An efficient and sensitive RP-HPLC method for detecting β-ODAP by pre-column derivatisation with O-phthalaldehyde (OPA) was developed in our laboratory. The usefulness of this method for measuring the β-ODAP content of thermally processed legume-based foods with various substitution levels of grasspea flour is demonstrated. The β-ODAP content of dehulled grasspea flour used was 5.26 ± 0.11 mg/g. The method shows a clear resolution between β-ODAP and its non-toxic α-isomer. At 1% (w/w) substitution it was possible to detect and distinguish the two isomers. Among the unit heat operations deep-frying results in the β-ODAP content being lowered by â¼80% irrespective of the substitution level. The decrease in β-ODAP content by fermentation was â¼58%, which was further decreased on steaming. The results indicate that levels as low as 0.01 mg of β-ODAP/g of product are measurable. Therefore this method is a useful tool in detecting the unwarranted use of grasspea flour in processed foods that pose health risks to unsuspecting customers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Food Composition and Analysis - Volume 22, Issues 7â8, NovemberâDecember 2009, Pages 704-708
Journal: Journal of Food Composition and Analysis - Volume 22, Issues 7â8, NovemberâDecember 2009, Pages 704-708
نویسندگان
Rohini Misra, Asha Martin, Lalitha R. Gowda,